methanosarcina_sp_medium_n2_co2_for_dsm_2834 (CultureMech:009174)

Overview

External ontology

TOGO:M2606 — Methanosarcina SP. Medium (N2/CO2) (for DSM 2834)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2606 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141c.pdf

Composition

flowchart LR
    CultureMech_009174["methanosarcina_sp_medium_n2_co2_for_dsm_2834"]
    ing0_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
    CultureMech_009174 --> ing0_Sodium_resazurin__0_1__w_v_
    ing1_Fe_NH4_2_SO4_2_x_6_H2O__0_1__w_v_["Fe(NH4)2(SO4)2 x 6 H2O (0.1% w/v)"]
    CultureMech_009174 --> ing1_Fe_NH4_2_SO4_2_x_6_H2O__0_1__w_v_
    CHEBI_76181["CHEBI:76181"]
    ing1_Fe_NH4_2_SO4_2_x_6_H2O__0_1__w_v_ -.-> CHEBI_76181
    ing2_Distilled_water["Distilled water"]
    CultureMech_009174 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009174 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_NaCl["NaCl"]
    CultureMech_009174 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009174 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_NH4Cl["NH4Cl"]
    CultureMech_009174 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_K2HPO4["K2HPO4"]
    CultureMech_009174 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_009174 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_009174 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_KCl["KCl"]
    CultureMech_009174 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_NaHCO3["NaHCO3"]
    CultureMech_009174 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na_acetate["Na-acetate"]
    CultureMech_009174 --> ing12_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing12_Na_acetate -.-> CHEBI_32954
    ing13_methanol["methanol"]
    CultureMech_009174 --> ing13_methanol
    CHEBI_17790["CHEBI:17790"]
    ing13_methanol -.-> CHEBI_17790
    ing14_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD BBL)"]
    CultureMech_009174 --> ing14_Trypticase_peptone__BD_BBL_
    ing15_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
    CultureMech_009174 --> ing15_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing15_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing16_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_009174 --> ing16_Yeast_extract__OXOID_
    ing17_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009174 --> ing17_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing17_Carbon_dioxide_gas -.-> CHEBI_16526
    ing18_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009174 --> ing18_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing18_Nitrogen_gas -.-> CHEBI_17997
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009174 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_H3BO3["H3BO3"]
    CultureMech_009174 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_009174 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_009174 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_009174 --> ing23_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing24_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_009174 --> ing24_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing24_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_009174 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_009174 --> ing26_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing27_AIK_SO4_2_x_12_H2O["AIK(SO4)2 x 12 H2O"]
    CultureMech_009174 --> ing27_AIK_SO4_2_x_12_H2O
    ing28_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_009174 --> ing28_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing28_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_009174 --> ing29_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
    CultureMech_009174 --> more["...12 more"]

Ingredients (42)

#	Ingredient	Concentration	Ontology	Notes
1	Sodium resazurin (0.1% w/v)	0.5 G_PER_L		Role: pH dependent redox indicator; Properties: Defined component, Solution
2	Fe(NH4)2(SO4)2 x 6 H2O (0.1% w/v) Fe.6H2O.2H4N.2O4S 392.139 g/mol	2 G_PER_L	CHEBI:76181 ferrous ammonium sulfate hexahydrate	Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
3	Distilled water H2O 18.015 g/mol	1000.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1000.0, 1000.0, 1000.0]
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	6.45 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.45, 3.0]
5	NaCl Cl.Na 58.443 g/mol	19.0 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 18.0, 1.0]
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.24000000000000002 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.14, 0.1]
7	NH4Cl Cl.H4N 53.492 g/mol	0.25 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
8	K2HPO4 HO4P.2K 174.174 g/mol	0.14 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	4 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
11	KCl Cl.K 74.551 g/mol	0.34 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12	NaHCO3 CHO3.Na 84.006 g/mol	5 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
13	Na-acetate C2H3O2.Na 82.034 g/mol	1 G_PER_L	CHEBI:32954 sodium acetate	Role: Buffer; Properties: Organic compound, Defined component, Simple component
14	methanol CH4O 32.042 g/mol	5 G_PER_L	CHEBI:17790 methanol	Role: Solvating media; Properties: Organic compound, Defined component, Simple component
15	Trypticase peptone (BD BBL)	2 G_PER_L		Properties: Undefined component
16	L-Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.5 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Properties: Organic compound, Simple component, Defined component
17	Yeast extract (OXOID)	2 G_PER_L		Properties: Complex component, Undefined component
18	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
19	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
20	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.01 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21	H3BO3 H3BO3 61.833 g/mol	0.01 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.03 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.18 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.01 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.3 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.18 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
28	AIK(SO4)2 x 12 H2O	0.02 G_PER_L		Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Role: Mineral source; Properties: Organic compound, Defined component, Simple component
30	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.4 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
31	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.5 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
32	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
33	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	5 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
34	Thiamine-HCl C12H17N4OS.Cl.HCl 337.276 g/mol	5 G_PER_L	CHEBI:49105 thiamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
35	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	10 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
36	Folic acid C19H19N7O6 441.404 g/mol	2 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
37	Vitamin B12	0.1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
38	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
39	Nicotinic acid C6H5NO2 123.111 g/mol	5 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
40	(DL)-alpha-Lipoic acid C8H14O2S2 206.332 g/mol	5 G_PER_L	CHEBI:16494 lipoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
41	Calcium D-(+)-pantothenate 2C9H16NO5.Ca 476.536 g/mol	5 G_PER_L	CHEBI:31345 Calcium pantothenate	Role: Vitamin; Properties: Defined component
42	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: Gas

Solutions

Modified Wolin's mineral solution

Dose: 10 G_PER_L

See mediadive_241_Modified_Wolin_s_mineral_solution.yaml for composition

Wolin's vitamin solution

Dose: 10 G_PER_L

See mediadive_242_Wolin_s_vitamin_solution.yaml for composition

KOH solution

Dose: variable VARIABLE

Role: Mineral source; Properties: Defined component, Solution

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.852051Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2606
2026-01-28T19:48:07.371027Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: KOH solution; Added default concentration for ingredient: N2
2026-02-06T05:10:12.658333Z	invalid-chebi-removal	Removed 1 invalid CHEBI ID(s)	Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-09T08:04:11.887663+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 3 solution(s) from ingredients
2026-03-13T03:08:38.675515Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 5 duplicate ingredient(s)
2026-03-13T06:20:03.623778Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009174
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:40.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.353897+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Methanosarcina SP. Medium (N2/CO2) (for DSM 2834)" to "methanosarcina_sp_medium_n2_co2_for_dsm_2834" for consistent matching
2026-05-16T09:04:22.599478+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:41:32.518083+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:47.909071+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24)	Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T06:58:23.361443+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:07.337920+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:30.876742+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.