Overview
- External ontology
- TOGO:M2610 — Carboxydothermus Medium (Heterotroph)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2610 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium508.pdf
Composition
flowchart LR
CultureMech_009178["carboxydothermus_medium_heterotroph"]
ing0_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
CultureMech_009178 --> ing0_Sodium_resazurin__0_1__w_v_
ing1_Distilled_water["Distilled water"]
CultureMech_009178 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Yeast_extract["Yeast extract"]
CultureMech_009178 --> ing2_Yeast_extract
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009178 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009178 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009178 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009178 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009178 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_KCl["KCl"]
CultureMech_009178 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Na2CO3["Na2CO3"]
CultureMech_009178 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_Na_pyruvate["Na-pyruvate"]
CultureMech_009178 --> ing10_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing10_Na_pyruvate -.-> CHEBI_50144
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009178 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_009178 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009178 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_009178 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009178 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009178 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009178 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009178 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009178 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009178 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_Na2_EDTA["Na2-EDTA"]
CultureMech_009178 --> ing21_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na2_EDTA -.-> CHEBI_64734
ing22_2_N_NaOH["2 N NaOH"]
CultureMech_009178 --> ing22_2_N_NaOH
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009178 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009178 --> ing24_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_pantothenate -.-> CHEBI_31345
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009178 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_009178 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_009178 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_009178 --> ing28_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing28_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing29_D____Biotin["D(+)-Biotin"]
CultureMech_009178 --> ing29_D____Biotin
CHEBI_15956["CHEBI:15956"]
ing29_D____Biotin -.-> CHEBI_15956
CultureMech_009178 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sodium resazurin (0.1% w/v) | 0.5 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 4 identical duplicates: 1000.0, 1000.0, 1000.0, 1000.0] |
| 3 | Yeast extract | 0.05 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.29 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.52 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
2.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 23 | 2 N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
130.0 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 80.0, 50.0] |
| 25 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
400.0 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 300.0, 100.0] |
| 27 | Vitamin B12 | 101.0 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 100.0, 1.0] |
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
250.0 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 200.0, 50.0] |
| 29 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
200 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 32 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
50 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Folic acid
C19H19N7O6 441.404 g/mol
|
20 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
50 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
50 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 37 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
50 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
Solutions
Trace element solution SL-4
Dose: 10 G_PER_L
See mediadive_2248_Trace_element_solution_SL-4.yaml for composition
Seven vitamins solution
Dose: 1 G_PER_L
See mediadive_4611_Seven_vitamins_solution.yaml for composition
Wolin's vitamin solution (10x)
Dose: 1 G_PER_L
See mediadive_5980_Wolin_s_vitamin_solution_10x.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.863768Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2610 |
| 2026-01-28T19:48:21.119148Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: 2 N NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2 |
| 2026-02-06T05:10:29.412007Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:34.993455+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:04.551889Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 8 duplicate ingredient(s) |
| 2026-03-13T06:20:03.686134Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009178 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.415527+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Carboxydothermus Medium (Heterotroph)" to "carboxydothermus_medium_heterotroph" for consistent matching |
| 2026-05-16T09:03:14.652462+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.924877+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.413301+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:47.240598+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |