reinforced_clostridial_medium_oxoid_cm149_with_sodium_lactate_60_solution_at_a_concentration_of_1_5 (CultureMech:009180)

Overview

External ontology

TOGO:M2613 — Reinforced Clostridial medium (Oxoid CM149) with sodium lactate (60% solution) at a concentration of 1.5%

Applications

Microbial cultivation

pH

7.0

Notes

TOGO M2613 imports ATCC Medium 1252 Reinforced Clostridial medium (Oxoid CM149) with sodium lactate. ATCC 1252 discloses 1.5% sodium lactate 60% solution and a final pH of 7.0; TOGO additionally lists 1000 ml distilled water and 38 g/L Reinforced Clostridial medium (Oxoid CM149). The commercial Oxoid base is retained as an opaque unmapped component because the source does not expand its composition.

Composition

flowchart LR
    CultureMech_009180["reinforced_clostridial_medium_oxoid_cm149_with_sodium_lactate_60_so..."]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009180 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Sodium_lactate__60__solution_["Sodium lactate (60% solution)"]
    CultureMech_009180 --> ing1_Sodium_lactate__60__solution_
    CHEBI_75228["CHEBI:75228"]
    ing1_Sodium_lactate__60__solution_ -.-> CHEBI_75228
    ing2_Reinforced_Clostridial_medium__Oxoid_CM149_["Reinforced Clostridial medium (Oxoid CM149)"]
    CultureMech_009180 --> ing2_Reinforced_Clostridial_medium__Oxoid_CM149_

Ingredients (3)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1.0 L	CHEBI:15377 water	TOGO M2613 lists 1000 ml distilled water; the import unit is corrected to 1 L.
2	Sodium lactate (60% solution) C3H5O3.Na 112.06 g/mol	1.5 PERCENT_W_V	CHEBI:75228 sodium lactate	ATCC Medium 1252 lists sodium lactate 60% solution at 1.5%.
3	Reinforced Clostridial medium (Oxoid CM149)	38.0 G_PER_L		TOGO M2613 lists 38 g/L commercial Oxoid CM149 Reinforced Clostridial Medium; ATCC 1252 names the same commercial base but does not disclose its component recipe.

Preparation

Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.870028Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2613
2026-01-28T19:48:15.596036Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:25.757532+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:20:03.702844Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009180
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:29:58.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.433041+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Reinforced Clostridial medium (Oxoid CM149) with sodium lactate (60% solution) at a concentration of 1.5%" to "reinforced_clostridial_medium_oxoid_cm149_with_sodium_lactate_60_solution_at_a_concentration_of_1_5" for consistent matching
2026-05-16T09:04:30.520907+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:41:44.147448+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00	repair_togo_m2613_rcm_lactate_score15.py	RESOLVED_TOGO_M2613_RCM_LACTATE_SCORE15	Corrected the TOGO M2613 water unit, flattened the sodium lactate solution into a grounded ingredient, added pH 7.0, and marked the commercial Oxoid CM149 base as an opaque unmapped component.