ignicoccus_medium

CultureMech:009181 archaea COMPLEX LIQUID
Original name: Ignicoccus Medium

Overview

External ontology
TOGO:M2614 — Ignicoccus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2614 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium897.pdf

Composition

flowchart LR
    CultureMech_009181["ignicoccus_medium"]
    ing0_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
    CultureMech_009181 --> ing0_Sodium_resazurin__0_1__w_v_
    ing1_Distilled_water["Distilled water"]
    CultureMech_009181 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009181 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_009181 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009181 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_009181 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_009181 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_009181 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_H3BO3["H3BO3"]
    CultureMech_009181 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_KCl["KCl"]
    CultureMech_009181 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009181 --> ing10__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing10__NH4_2SO4 -.-> CHEBI_62946
    ing11_Sulfur__powdered_["Sulfur (powdered)"]
    CultureMech_009181 --> ing11_Sulfur__powdered_
    ing12_NaBr["NaBr"]
    CultureMech_009181 --> ing12_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing12_NaBr -.-> CHEBI_63004
    ing13_Meat_extract["Meat extract"]
    CultureMech_009181 --> ing13_Meat_extract
    ing14_2_N_H2SO4["2 N H2SO4"]
    CultureMech_009181 --> ing14_2_N_H2SO4
    ing15_N2["N2"]
    CultureMech_009181 --> ing15_N2
    CHEBI_17997["CHEBI:17997"]
    ing15_N2 -.-> CHEBI_17997
    ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009181 --> ing16_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing16_Carbon_dioxide_gas -.-> CHEBI_16526
    ing17_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009181 --> ing17_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing17_Hydrogen_gas -.-> CHEBI_18276

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 Sodium resazurin (0.1% w/v) 0.5 G_PER_L Role: pH dependent redox indicator; Properties: Solution, Defined component
2 Distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 NaCl
Cl.Na 58.443 g/mol
13.65 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.38 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
6 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
Role: Reducing agent; Properties: Defined component, Inorganic compound, Simple component
9 H3BO3
H3BO3 61.833 g/mol
15 G_PER_L CHEBI:33118
boric acid
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.25 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
12 Sulfur (powdered) 5 G_PER_L Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
13 NaBr
Br.Na 102.894 g/mol
0.05 G_PER_L CHEBI:63004
sodium bromide
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
14 Meat extract 1 G_PER_L Properties: Undefined component
15 2 N H2SO4 variable VARIABLE Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
16 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: Gas
17 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
18 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
Properties: ガス

Solutions

SrCl3 x 6 H2O solution (0.1% w/v)

Dose: 7 G_PER_L

Role: Mineral source; Properties: Solution, Defined component

KI solution (0.01% w/v)

Dose: 0.5 G_PER_L

Role: Mineral source; Properties: Solution, Defined component, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.870972Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2614
2026-01-28T19:48:20.817682Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: 2 N H2SO4; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:34.524407+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:03.711469Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009181
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.441315+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Ignicoccus Medium" to "ignicoccus_medium" for consistent matching
2026-05-16T09:03:14.677003+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:37.949042+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:27.558642+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:49.426387+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.