Overview
- External ontology
- TOGO:M2615 — Desulfovibrio (Postgate) Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2615 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63.pdf
Composition
flowchart LR
CultureMech_009182["desulfovibrio_postgate_medium"]
ing0_N2_gas["N2 gas"]
CultureMech_009182 --> ing0_N2_gas
ing1_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
CultureMech_009182 --> ing1_Sodium_resazurin__0_1__w_v_
ing2_Distilled_water["Distilled water"]
CultureMech_009182 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009182 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_009182 --> ing4_Yeast_extract
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009182 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_NH4Cl["NH4Cl"]
CultureMech_009182 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_K2HPO4["K2HPO4"]
CultureMech_009182 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Na2SO4["Na2SO4"]
CultureMech_009182 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_Na_DL_lactate["Na-DL-lactate"]
CultureMech_009182 --> ing9_Na_DL_lactate
CHEBI_75228["CHEBI:75228"]
ing9_Na_DL_lactate -.-> CHEBI_75228
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009182 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_Ascorbic_acid["Ascorbic acid"]
CultureMech_009182 --> ing11_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing11_Ascorbic_acid -.-> CHEBI_22652
ing12_Na_thioglycolate["Na-thioglycolate"]
CultureMech_009182 --> ing12_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing12_Na_thioglycolate -.-> CHEBI_86481
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | N2 gas | variable VARIABLE | Properties: ガス | |
| 2 | Sodium resazurin (0.1% w/v) | 0.5 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 3 duplicates: 980.0, 10.0, 10.0] |
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
Na-DL-lactate
C3H5O3.Na 112.06 g/mol
|
2 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 12 | Ascorbic acid | 0.1 G_PER_L |
CHEBI:22652
ascorbic acid
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 13 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.1 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Solution A
Dose: 980 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 10 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 10 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
NaOH solution
Dose: variable VARIABLE
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.873291Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2615 |
| 2026-01-28T19:48:34.333924Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: NaOH solution; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:56.996325+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:28.572371Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:03.722603Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009182 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.452018+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfovibrio (Postgate) Medium" to "desulfovibrio_postgate_medium" for consistent matching |
| 2026-05-16T09:03:14.688939+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.961192+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.570933+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.431495+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |