Overview
- External ontology
- TOGO:M2622 — BHI w/ heat-inactivated Fetal Bovine Serum and Glucose
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2622 Original URL: https://www.atcc.org/~/media/6961826D153043B3A8D59D0D37CEF5C8.ashx Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009188["bhi_w_heat_inactivated_fetal_bovine_serum_and_glucose"]
ing0_DI_Water["DI Water"]
CultureMech_009188 --> ing0_DI_Water
ing1_Calf_brains["Calf brains"]
CultureMech_009188 --> ing1_Calf_brains
ing2_Beef_heart["Beef heart"]
CultureMech_009188 --> ing2_Beef_heart
ing3_Proteose_peptone["Proteose peptone"]
CultureMech_009188 --> ing3_Proteose_peptone
ing4_Dextrose["Dextrose"]
CultureMech_009188 --> ing4_Dextrose
CHEBI_17634["CHEBI:17634"]
ing4_Dextrose -.-> CHEBI_17634
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_009188 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Disodium_phosphate["Disodium phosphate"]
CultureMech_009188 --> ing6_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing6_Disodium_phosphate -.-> CHEBI_34683
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009188 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_009188 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_Hydrogen_gas["Hydrogen gas"]
CultureMech_009188 --> ing9_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing9_Hydrogen_gas -.-> CHEBI_18276
ing10_Heat_inactivated_fetal_bovine_serum["Heat inactivated fetal bovine serum"]
CultureMech_009188 --> ing10_Heat_inactivated_fetal_bovine_serum
ing11_Glucose["Glucose"]
CultureMech_009188 --> ing11_Glucose
CHEBI_17234["CHEBI:17234"]
ing11_Glucose -.-> CHEBI_17234
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 900.0 G_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 850.0, 50.0] | |
| 2 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 5 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 7 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 11 | Heat inactivated fetal bovine serum | 100 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 12 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
Solutions
Glucose Solution
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.882747Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2622 |
| 2026-01-28T19:48:22.875062Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:38.056586+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:07.809607Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.765340Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009188 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.493878+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHI w/ heat-inactivated Fetal Bovine Serum and Glucose" to "bhi_w_heat_inactivated_fetal_bovine_serum_and_glucose" for consistent matching |
| 2026-05-16T09:03:25.773748+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:50.938206+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |