Overview
- External ontology
- TOGO:M2635 — RGCA Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2635 Original URL: https://www.atcc.org/~/media/0F7D3D7F1046495DAE3216BB2B40AC29.ashx
Composition
flowchart LR
CultureMech_009192["rgca_medium"]
ing0_Rumen_Fluid["Rumen Fluid"]
CultureMech_009192 --> ing0_Rumen_Fluid
ing1_Agar["Agar"]
CultureMech_009192 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_CO2_gas["CO2 gas"]
CultureMech_009192 --> ing2_CO2_gas
ing3_K2HPO4["K2HPO4"]
CultureMech_009192 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_DI_Water["DI Water"]
CultureMech_009192 --> ing4_DI_Water
ing5_NaCl["NaCl"]
CultureMech_009192 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_KH2PO4["KH2PO4"]
CultureMech_009192 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_009192 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_CaCl2["CaCl2"]
CultureMech_009192 --> ing8_CaCl2
CHEBI_3312["CHEBI:3312"]
ing8_CaCl2 -.-> CHEBI_3312
ing9_MgSO4["MgSO4"]
CultureMech_009192 --> ing9_MgSO4
CHEBI_32599["CHEBI:32599"]
ing9_MgSO4 -.-> CHEBI_32599
ing10_Resazurin["Resazurin"]
CultureMech_009192 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Cellobiose["Cellobiose"]
CultureMech_009192 --> ing11_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing11_Cellobiose -.-> CHEBI_17057
ing12_Glucose["Glucose"]
CultureMech_009192 --> ing12_Glucose
CHEBI_17234["CHEBI:17234"]
ing12_Glucose -.-> CHEBI_17234
ing13_CysteineHCl["CysteineHCl"]
CultureMech_009192 --> ing13_CysteineHCl
ing14_Na2CO3["Na2CO3"]
CultureMech_009192 --> ing14_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing14_Na2CO3 -.-> CHEBI_29377
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rumen Fluid | 400 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 3 | CO2 gas | variable VARIABLE | Properties: ガス | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 | DI Water | 1500.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 500.0, 500.0, 50.0, 325.0, 25.0, 100.0] | |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
3 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.3 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.3 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.05 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 12 |
Cellobiose
C12H22O11 342.297 g/mol
|
3 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 13 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 14 | CysteineHCl | 0.75 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 15 |
Na2CO3
CO3.2Na 105.988 g/mol
|
6 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution 1
Dose: 150 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Solution 2
Dose: 150 G_PER_L
See mediadive_6109_Solution_2.yaml for composition
Solution 3
Dose: 1 G_PER_L
See mediadive_6110_Solution_3.yaml for composition
Solution 4
Dose: 220 G_PER_L
See mediadive_5181_Solution_4.yaml for composition
Solution 5
Dose: 17 G_PER_L
See mediadive_5183_Solution_5.yaml for composition
Solution 6
Dose: 66 G_PER_L
See mediadive_5184_Solution_6.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.893509Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2635 |
| 2026-01-28T19:48:34.468094Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 gas; Added default concentration for ingredient: CO2 gas; Added default concentration for ingredient: CO2 gas |
| 2026-03-09T08:04:57.245816+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:28.824858Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:20:03.826079Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009192 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.554244+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RGCA Medium" to "rgca_medium" for consistent matching |
| 2026-05-16T09:04:30.554464+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:44.185793+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.897714+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |