Overview
- External ontology
- TOGO:M2636 — Ruminococcus Albus Medium + maltose (1g/L), starch (1g/L)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2636 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium436.pdf Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preparation order is preserved as source text; provider paragraph grouping does not make all inputs simultaneous: Boil and cool under CO2. Under a gentle stream of CO2 add: Adjust pH to 7.0, distribute under N2 into appropriate vessels and sterilize. Inoculate under CO2 and incubate at 37oC. Original-source cross-check retains source-specific preparation stages and calcium hydrate labels. Similar names do not establish exact equivalence, percentage basis or a final-volume denominator. Complete source rows determine the reconstructed formulation. Archive the prior flattened nodes, unsupported LB additions and stock links in append-only history. Unrelated annotations are preserved, not recertified. Complete source rows determine the reconstructed formulation. Archive the prior flattened nodes, unsupported LB additions and stock links in append-only history. Unrelated annotations are preserved, not recertified.
Composition
flowchart LR
CultureMech_009193["ruminococcus_albus_medium_maltose_1g_l_starch_1g_l"]
ing0_Distilled_water["Distilled water"]
CultureMech_009193 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009193 --> ing1_Yeast_extract
ing2_Resazurin["Resazurin"]
CultureMech_009193 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Cellobiose["Cellobiose"]
CultureMech_009193 --> ing3_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing3_Cellobiose -.-> CHEBI_17057
ing4_Glucose["Glucose"]
CultureMech_009193 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Tryptone["Tryptone"]
CultureMech_009193 --> ing5_Tryptone
ing6_CO2["CO2"]
CultureMech_009193 --> ing6_CO2
CHEBI_16526["CHEBI:16526"]
ing6_CO2 -.-> CHEBI_16526
ing7_Na2CO3["Na2CO3"]
CultureMech_009193 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
ing8_Starch["Starch"]
CultureMech_009193 --> ing8_Starch
CHEBI_28017["CHEBI:28017"]
ing8_Starch -.-> CHEBI_28017
ing9_Maltose["Maltose"]
CultureMech_009193 --> ing9_Maltose
CHEBI_18167["CHEBI:18167"]
ing9_Maltose -.-> CHEBI_18167
ing10_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_009193 --> ing10_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_N2["N2"]
CultureMech_009193 --> ing11_N2
CHEBI_17997["CHEBI:17997"]
ing11_N2 -.-> CHEBI_17997
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
0.92 L |
CHEBI:15377
water
|
Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 920 ml at components[0].items[0]. |
| 2 | Yeast extract | Role: Nutrient source; Properties: Undefined component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 2 g at components[0].items[1]. | ||
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 1 mg at components[0].items[2]. | |
| 4 |
Cellobiose
C12H22O11 342.297 g/mol
|
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 2 g at components[0].items[3]. | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 3 g at components[0].items[4]. | |
| 6 | Tryptone | Properties: Undefined component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 5 g at components[0].items[5]. | ||
| 7 |
CO2
CO2 44.009 g/mol
|
CHEBI:16526
carbon dioxide
|
Properties: ガス No numeric source dose; never turn missing into zero Source input: None None at components[0].items[8]. | |
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 4 g at components[1].items[0]. | |
| 9 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Organic compound, Defined component, Complex component This exact variant title explicitly gives each supplement rate; this is not a denominator for other batch ingredients Source input: 1 g at components[1].items[1]. Additional exact excerpt at meta.name: Ruminococcus Albus Medium + maltose (1g/L), starch (1g/L) |
| 10 |
Maltose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component This exact variant title explicitly gives each supplement rate; this is not a denominator for other batch ingredients Source input: 1 g at components[1].items[2]. Additional exact excerpt at meta.name: Ruminococcus Albus Medium + maltose (1g/L), starch (1g/L) |
| 11 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component Keep source mass and preparation stage in evidenced notes, not g/L Source input: 500 mg at components[1].items[3]. | |
| 12 |
N2
N2 28.014 g/mol
|
CHEBI:17997
dinitrogen
|
Properties: ガス No numeric source dose; never turn missing into zero Source input: None None at components[1].items[5]. |
Solutions
Mineral solution 1
Dose: 0.04 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.1 L | Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 100 ml at components[2].items[0]. |
| K2HPO4 | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Keep literal percentage in source-backed notes; no w/v, w/w or v/v assignment Source input: 0.6 % at components[2].items[1]. |
Source input only; not final volume or percentage Source input: 40 ml at components[0].items[6].
Mineral solution 2
Dose: 0.04 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.1 L | Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 100 ml at components[3].items[0]. |
| MgSO4 x 7 H2O | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Keep literal percentage in source-backed notes; no w/v, w/w or v/v assignment Source input: 0.25 % at components[3].items[1]. | |
| NaCl | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Keep literal percentage in source-backed notes; no w/v, w/w or v/v assignment Source input: 1.2 % at components[3].items[2]. | |
| KH2PO4 | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component Keep literal percentage in source-backed notes; no w/v, w/w or v/v assignment Source input: 0.6 % at components[3].items[3]. | |
| (NH4)2SO4 | Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component Keep literal percentage in source-backed notes; no w/v, w/w or v/v assignment Source input: 2 % at components[3].items[4]. | |
| CaCl2 x 7 H2O | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component Keep literal percentage in source-backed notes; no w/v, w/w or v/v assignment Source input: 0.16 % at components[3].items[5]. Retain the literal seven-water source label. Other sources use dihydrate or hexahydrate labels; no chemical-form equivalence or molecular weight is inferred. Existing generic calcium-chloride annotation is not hydrate-specific evidence. |
Source input only; not final volume or percentage Source input: 40 ml at components[0].items[7].
The old solution4674 reference described a different formula (dibasic phosphate, different salts and hydrate). This source's inline percent-labelled stock supersedes that link; no same-name substitution is permitted.
Fatty acid mixture
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Distilled water | 0.07 L | Superseded flattening history, not a current amount: Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 920.0, 100.0, 100.0, 70.0] Source input only; not final volume or percentage Source input: 70 ml at components[4].items[0]. |
| Isovaleric acid | 0.01 L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source input only; not final volume or percentage Source input: 10 ml at components[4].items[1]. |
| Isobutyric acid | 0.01 L | Role: Carbon source; Properties: Organic compound, Defined component, Simple component Source input only; not final volume or percentage Source input: 10 ml at components[4].items[2]. |
| 2-Methylbutyric acid | 0.01 L | Source input only; not final volume or percentage Source input: 10 ml at components[4].items[3]. |
Source input only; not final volume or percentage Source input: 1 ml at components[1].items[4].
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.896317Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2636 |
| 2026-01-28T19:48:12.335719Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:20.360173+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:48.359398Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:03.839313Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009193 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.567519+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ruminococcus Albus Medium + maltose (1g/L), starch (1g/L)" to "ruminococcus_albus_medium_maltose_1g_l_starch_1g_l" for consistent matching |
| 2026-05-16T09:04:31.014322+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:44.877997+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:31.539143+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:11.159632+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T07:30:35.561516+00:00 | codex | RESTORED_RUMINOCOCCUS_ALBUS_SOURCE_FORMULATIONS_WITH_EVIDENCE | Restore exact-source medium436 formulation, preparation stages and input units. Preserve qualified prior rates, source-specific nulls and unresolved original bases. No growth association added. |