Overview
- External ontology
- TOGO:M263 — Gnys Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M263 Original source: JCM - JCM_M270-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=270
Composition
flowchart LR
CultureMech_009197["gnys_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009197 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009197 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009197 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009197 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009197 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_009197 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_agar["agar"]
CultureMech_009197 --> ing6_agar
CHEBI_2509["CHEBI:2509"]
ing6_agar -.-> CHEBI_2509
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009197 --> ing7_Yeast_extract__BD_Difco_
ing8_Sodium_gluconate["Sodium gluconate"]
CultureMech_009197 --> ing8_Sodium_gluconate
CHEBI_84997["CHEBI:84997"]
ing8_Sodium_gluconate -.-> CHEBI_84997
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | agar | 18 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 9 |
Sodium gluconate
C6H11O7.Na 218.137 g/mol
|
3 G_PER_L |
CHEBI:84997
sodium gluconate
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Trace element solution SL8 (see Medium [M183])
Dose: 1 G_PER_L
Cross-reference to Medium M183
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.724561Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M263 |
| 2026-01-28T19:48:24.499176Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.499841+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:03.878106Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009197 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.608026+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Gnys Medium" to "gnys_medium" for consistent matching |
| 2026-05-16T09:03:14.776317+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:38.052014+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.465808+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |