Overview
- External ontology
- TOGO:M2642 — MS-OCM Base Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2642 Original URL: https://www.atcc.org/~/media/E6135A7852C143D9BBC635D4964700AE.ashx
Composition
flowchart LR
CultureMech_009199["ms_ocm_base_medium"]
ing0_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_009199 --> ing0_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing0_Sodium_bicarbonate -.-> CHEBI_32139
ing1_DI_Water["DI Water"]
CultureMech_009199 --> ing1_DI_Water
ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009199 --> ing2_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing2_Carbon_dioxide_gas -.-> CHEBI_16526
ing3_Nitrogen_gas["Nitrogen gas"]
CultureMech_009199 --> ing3_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing3_Nitrogen_gas -.-> CHEBI_17997
ing4_Yeast_extract["Yeast extract"]
CultureMech_009199 --> ing4_Yeast_extract
ing5_Trypticase_peptone["Trypticase peptone"]
CultureMech_009199 --> ing5_Trypticase_peptone
ing6_Trace_Mineral_Supplement__ATCC_MD_TMS["Trace Mineral Supplement, ATCC MD-TMS"]
CultureMech_009199 --> ing6_Trace_Mineral_Supplement__ATCC_MD_TMS
ing7_Ammonium_chloride["Ammonium chloride"]
CultureMech_009199 --> ing7_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing7_Ammonium_chloride -.-> CHEBI_31206
ing8_Magnesium_chloride_hexahydrate["Magnesium chloride hexahydrate"]
CultureMech_009199 --> ing8_Magnesium_chloride_hexahydrate
CHEBI_86345["CHEBI:86345"]
ing8_Magnesium_chloride_hexahydrate -.-> CHEBI_86345
ing9_Calcium_chloride_dehydrate["Calcium chloride dehydrate"]
CultureMech_009199 --> ing9_Calcium_chloride_dehydrate
ing10_Potassium_dibasic_phosphate_trihydrate["Potassium dibasic phosphate trihydrate"]
CultureMech_009199 --> ing10_Potassium_dibasic_phosphate_trihydrate
ing11_Resazurin["Resazurin"]
CultureMech_009199 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_Distilled_Water["Distilled Water"]
CultureMech_009199 --> ing12_Distilled_Water
CHEBI_15377["CHEBI:15377"]
ing12_Distilled_Water -.-> CHEBI_15377
ing13_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009199 --> ing13_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4___7H2O -.-> CHEBI_31795
ing14_NaCl["NaCl"]
CultureMech_009199 --> ing14_NaCl
CHEBI_26710["CHEBI:26710"]
ing14_NaCl -.-> CHEBI_26710
ing15_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009199 --> ing15_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4___2H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_009199 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009199 --> ing17_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4___7H2O -.-> CHEBI_75836
ing18_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009199 --> ing18_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2___6H2O -.-> CHEBI_34887
ing19_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009199 --> ing19_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4___7H2O -.-> CHEBI_32312
ing20_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009199 --> ing20_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4___5H2O -.-> CHEBI_31440
ing21_Na2WO4___2H2O["Na2WO4 . 2H2O"]
CultureMech_009199 --> ing21_Na2WO4___2H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4___2H2O -.-> CHEBI_63939
ing22_CaCl2__anhydrous_["CaCl2 (anhydrous)"]
CultureMech_009199 --> ing22_CaCl2__anhydrous_
ing23_MnSO4___H2O["MnSO4 . H2O"]
CultureMech_009199 --> ing23_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing23_MnSO4___H2O -.-> CHEBI_86364
ing24_AlK_SO4_2__anhydrous_["AlK(SO4)2 (anhydrous)"]
CultureMech_009199 --> ing24_AlK_SO4_2__anhydrous_
ing25_Na2SeO3__anhydrous_["Na2SeO3 (anhydrous)"]
CultureMech_009199 --> ing25_Na2SeO3__anhydrous_
ing26_Co_NO3_2___6H2O["Co(NO3)2 . 6H2O"]
CultureMech_009199 --> ing26_Co_NO3_2___6H2O
CHEBI_86214["CHEBI:86214"]
ing26_Co_NO3_2___6H2O -.-> CHEBI_86214
ing27_EDTA["EDTA"]
CultureMech_009199 --> ing27_EDTA
CHEBI_4735["CHEBI:4735"]
ing27_EDTA -.-> CHEBI_4735
ing28_Mercaptoethanesulfonic_acid["Mercaptoethanesulfonic acid"]
CultureMech_009199 --> ing28_Mercaptoethanesulfonic_acid
ing29_N2_gas["N2 gas"]
CultureMech_009199 --> ing29_N2_gas
CultureMech_009199 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
8.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 2 | DI Water | 4276.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 976.0, 1000.0, 1000.0, 1000.0, 100.0, 100.0, 100.0] | |
| 3 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 4 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 5 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 | Trypticase peptone | 2 G_PER_L | Properties: Undefined component | |
| 7 | Trace Mineral Supplement, ATCC MD-TMS | 10 G_PER_L | ||
| 8 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
100 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Magnesium chloride hexahydrate
2Cl.6H2O.Mg 203.301 g/mol
|
100 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Calcium chloride dehydrate | 40 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 11 | Potassium dibasic phosphate trihydrate | 200 G_PER_L | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component | |
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 13 |
Distilled Water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2WO4 . 2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 | CaCl2 (anhydrous) | 0.1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 24 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 | AlK(SO4)2 (anhydrous) | 0.01 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 26 | Na2SeO3 (anhydrous) | 0.001 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 27 |
Co(NO3)2 . 6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.1 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
| 29 | Mercaptoethanesulfonic acid | 5 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 30 | N2 gas | variable VARIABLE | Properties: ガス | |
| 31 |
Sodium sulfide nonahydrate
9H2O.2Na.S 240.182 g/mol
|
1.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
Cysteine
C3H7NO2S 121.161 g/mol
|
3 G_PER_L |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 10 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 2 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 2 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Co-enzyme M (mercaptoethanesulfonic acid) Solution (100 X solution)
Dose: 10 G_PER_L
HCl solution
Dose: variable VARIABLE
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.911219Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2642 |
| 2026-01-28T19:48:19.724897Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: HCl solution; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:32.674442+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:01.964239Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:20:03.915595Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009199 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.643827+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MS-OCM Base Medium" to "ms_ocm_base_medium" for consistent matching |
| 2026-05-16T09:04:26.926601+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:38.716541+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:27.480872+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.223746+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |