hs_medium_methanol_acetate_medium_125_and_40_mm_respectively

CultureMech:009200 bacterial COMPLEX LIQUID
Original name: HS medium-methanol-acetate medium (125 and 40 mM, respectively)

Overview

External ontology
TOGO:M2643 — HS medium-methanol-acetate medium (125 and 40 mM, respectively)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2643

Composition

flowchart LR
    CultureMech_009200["hs_medium_methanol_acetate_medium_125_and_40_mm_respectively"]
    ing0_methanol["methanol"]
    CultureMech_009200 --> ing0_methanol
    CHEBI_17790["CHEBI:17790"]
    ing0_methanol -.-> CHEBI_17790
    ing1_acetate["acetate"]
    CultureMech_009200 --> ing1_acetate
    CHEBI_30089["CHEBI:30089"]
    ing1_acetate -.-> CHEBI_30089
    ing2_HS_medium["HS medium"]
    CultureMech_009200 --> ing2_HS_medium
    ing3_Distilled_Water["Distilled Water"]
    CultureMech_009200 --> ing3_Distilled_Water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_Water -.-> CHEBI_15377
    ing4_NaCl["NaCl"]
    CultureMech_009200 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_KH2PO4["KH2PO4"]
    CultureMech_009200 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_009200 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_resazurin["resazurin"]
    CultureMech_009200 --> ing7_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_resazurin -.-> CHEBI_8806
    ing8_MgCl2___6H2O["MgCl2 . 6H2O"]
    CultureMech_009200 --> ing8_MgCl2___6H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2___6H2O -.-> CHEBI_86345
    ing9_Na2S___9H2O["Na2S . 9H2O"]
    CultureMech_009200 --> ing9_Na2S___9H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S___9H2O -.-> CHEBI_76209
    ing10_KCl["KCl"]
    CultureMech_009200 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_NaHCO3["NaHCO3"]
    CultureMech_009200 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_CaCl2["CaCl2"]
    CultureMech_009200 --> ing12_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing12_CaCl2 -.-> CHEBI_3312
    ing13_cysteine___HCl["cysteine . HCl"]
    CultureMech_009200 --> ing13_cysteine___HCl

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 methanol
CH4O 32.042 g/mol
125 MILLIMOLAR CHEBI:17790
methanol
 Role: Solvating media; Properties: Defined component, Organic compound, Simple component
2 acetate
C2H3O2 59.044 g/mol
40 MILLIMOLAR CHEBI:30089
acetate
 Role: Carbon source; Properties: Simple component, Defined component, Organic compound
3 HS medium 1 G_PER_L
4 Distilled Water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
5 NaCl
Cl.Na 58.443 g/mol
0.4 MOLAR CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 KH2PO4
H2O4P.K 136.084 g/mol
5 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7 NH4Cl
Cl.H4N 53.492 g/mol
19 MILLIMOLAR CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
8 resazurin
C12H7NO4 229.191 g/mol
4 MICROMOLAR CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
9 MgCl2 . 6H2O
2Cl.6H2O.Mg 203.301 g/mol
54 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
0.4 MILLIMOLAR CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Simple component, Defined component
11 KCl
Cl.K 74.551 g/mol
13 MILLIMOLAR CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
12 NaHCO3
CHO3.Na 84.006 g/mol
45 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
13 CaCl2
Ca.2Cl 110.984 g/mol
2 MILLIMOLAR CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
14 cysteine . HCl 2.8 MILLIMOLAR Properties: Defined component, Organic compound, Simple component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.915791Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2643
2026-01-28T19:48:10.082144Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:03.926804Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009200
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.655330+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HS medium-methanol-acetate medium (125 and 40 mM, respectively)" to "hs_medium_methanol_acetate_medium_125_and_40_mm_respectively" for consistent matching
2026-06-05T07:41:08.759362+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.828917+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.