Overview
- External ontology
- TOGO:M2646 — MH-OCM Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2646 Original URL: https://www.atcc.org/~/media/B8879419AA034A51BD3BD6773490AEDE.ashx
Composition
flowchart LR
CultureMech_009202["mh_ocm_medium"]
ing0_Distilled_Water["Distilled Water"]
CultureMech_009202 --> ing0_Distilled_Water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_Water -.-> CHEBI_15377
ing1_Sodium_bicarbonate["Sodium bicarbonate"]
CultureMech_009202 --> ing1_Sodium_bicarbonate
CHEBI_32139["CHEBI:32139"]
ing1_Sodium_bicarbonate -.-> CHEBI_32139
ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009202 --> ing2_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing2_Carbon_dioxide_gas -.-> CHEBI_16526
ing3_Nitrogen_gas["Nitrogen gas"]
CultureMech_009202 --> ing3_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing3_Nitrogen_gas -.-> CHEBI_17997
ing4_Yeast_extract["Yeast extract"]
CultureMech_009202 --> ing4_Yeast_extract
ing5_NaCl["NaCl"]
CultureMech_009202 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_KCl["KCl"]
CultureMech_009202 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_MgCl2_x_7H2O["MgCl2 x 7H2O"]
CultureMech_009202 --> ing7_MgCl2_x_7H2O
CHEBI_6636["CHEBI:6636"]
ing7_MgCl2_x_7H2O -.-> CHEBI_6636
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_009202 --> ing8_Trypticase_peptone
ing9_Trace_Mineral_Supplement__Catalog_No__MD_TMS["Trace Mineral Supplement, Catalog No. MD-TMS"]
CultureMech_009202 --> ing9_Trace_Mineral_Supplement__Catalog_No__MD_TMS
ing10_Ammonium_chloride["Ammonium chloride"]
CultureMech_009202 --> ing10_Ammonium_chloride
CHEBI_31206["CHEBI:31206"]
ing10_Ammonium_chloride -.-> CHEBI_31206
ing11_Magnesium_chloride_hexahydrate["Magnesium chloride hexahydrate"]
CultureMech_009202 --> ing11_Magnesium_chloride_hexahydrate
CHEBI_86345["CHEBI:86345"]
ing11_Magnesium_chloride_hexahydrate -.-> CHEBI_86345
ing12_Calcium_chloride_dehydrate["Calcium chloride dehydrate"]
CultureMech_009202 --> ing12_Calcium_chloride_dehydrate
ing13_Potassium_dibasic_phosphate_trihydrate["Potassium dibasic phosphate trihydrate"]
CultureMech_009202 --> ing13_Potassium_dibasic_phosphate_trihydrate
ing14_Resazurin["Resazurin"]
CultureMech_009202 --> ing14_Resazurin
CHEBI_8806["CHEBI:8806"]
ing14_Resazurin -.-> CHEBI_8806
ing15_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009202 --> ing15_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing15_MgSO4___7H2O -.-> CHEBI_31795
ing16_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009202 --> ing16_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4___2H2O -.-> CHEBI_75213
ing17_H3BO3["H3BO3"]
CultureMech_009202 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009202 --> ing18_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4___7H2O -.-> CHEBI_75836
ing19_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009202 --> ing19_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2___6H2O -.-> CHEBI_34887
ing20_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009202 --> ing20_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4___7H2O -.-> CHEBI_32312
ing21_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009202 --> ing21_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4___5H2O -.-> CHEBI_31440
ing22_Na2WO4___2H2O["Na2WO4 . 2H2O"]
CultureMech_009202 --> ing22_Na2WO4___2H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4___2H2O -.-> CHEBI_63939
ing23_CaCl2__anhydrous_["CaCl2 (anhydrous)"]
CultureMech_009202 --> ing23_CaCl2__anhydrous_
ing24_MnSO4___H2O["MnSO4 . H2O"]
CultureMech_009202 --> ing24_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing24_MnSO4___H2O -.-> CHEBI_86364
ing25_AlK_SO4_2__anhydrous_["AlK(SO4)2 (anhydrous)"]
CultureMech_009202 --> ing25_AlK_SO4_2__anhydrous_
ing26_Na2SeO3__anhydrous_["Na2SeO3 (anhydrous)"]
CultureMech_009202 --> ing26_Na2SeO3__anhydrous_
ing27_Co_NO3_2___6H2O["Co(NO3)2 . 6H2O"]
CultureMech_009202 --> ing27_Co_NO3_2___6H2O
CHEBI_86214["CHEBI:86214"]
ing27_Co_NO3_2___6H2O -.-> CHEBI_86214
ing28_EDTA["EDTA"]
CultureMech_009202 --> ing28_EDTA
CHEBI_4735["CHEBI:4735"]
ing28_EDTA -.-> CHEBI_4735
ing29_Co_enzyme_M__mercaptoethanesulfonic_acid__["Co-enzyme M (mercaptoethanesulfonic acid) "]
CultureMech_009202 --> ing29_Co_enzyme_M__mercaptoethanesulfonic_acid__
CultureMech_009202 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled Water
H2O 18.015 g/mol
|
1289.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 8 duplicates: 976.0, 1.0, 1.0, 1.0, 10.0, 100.0, 100.0, 100.0] |
| 2 |
Sodium bicarbonate
CHO3.Na 84.006 g/mol
|
8.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 4 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 5 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
88.75 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 87.75, 1.0] |
| 7 |
KCl
Cl.K 74.551 g/mol
|
1.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MgCl2 x 7H2O
2Cl.Mg 95.211 g/mol
|
5 G_PER_L |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | Trypticase peptone | 2 G_PER_L | Properties: Undefined component | |
| 10 | Trace Mineral Supplement, Catalog No. MD-TMS | 10 G_PER_L | ||
| 11 |
Ammonium chloride
Cl.H4N 53.492 g/mol
|
100 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Magnesium chloride hexahydrate
2Cl.6H2O.Mg 203.301 g/mol
|
100 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Calcium chloride dehydrate | 40 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 14 | Potassium dibasic phosphate trihydrate | 200 G_PER_L | Role: Buffer; Properties: Inorganic compound, Defined component, Simple component | |
| 15 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 16 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2WO4 . 2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 | CaCl2 (anhydrous) | 0.1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 25 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 | AlK(SO4)2 (anhydrous) | 0.01 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 27 | Na2SeO3 (anhydrous) | 0.001 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 28 |
Co(NO3)2 . 6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.1 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
| 30 | Co-enzyme M (mercaptoethanesulfonic acid) | 5 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 31 | N2 gas | variable VARIABLE | Properties: ガス | |
| 32 |
Sodium sulfide
2Na.S 78.047 g/mol
|
1.5 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
Cysteine
C3H7NO2S 121.161 g/mol
|
3 G_PER_L |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 10 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 2 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 2 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Co-enzyme M (mercaptoethanesulfonic acid) (100 X solution):
Dose: 10 G_PER_L
HCl solution
Dose: variable VARIABLE
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.922989Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2646 |
| 2026-01-28T19:48:38.064889Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: HCl solution; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:05:03.630247+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:35.557555Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 8 duplicate ingredient(s) |
| 2026-03-13T06:20:03.964646Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009202 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.692179+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MH-OCM Medium" to "mh_ocm_medium" for consistent matching |
| 2026-05-16T09:04:23.376580+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:33.691113+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:24.105838+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:07.771805+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |