Overview
- External ontology
- TOGO:M2649 — Anaerobic Freshwater (FWM) Medium (for DSM 15978)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2649 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf
Composition
flowchart LR
CultureMech_009205["anaerobic_freshwater_fwm_medium_for_dsm_15978"]
ing0_Distilled_water["Distilled water"]
CultureMech_009205 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009205 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009205 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009205 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009205 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009205 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009205 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009205 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_009205 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009205 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_009205 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009205 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009205 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009205 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009205 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_ZnCl2["ZnCl2"]
CultureMech_009205 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
CultureMech_009205 --> ing16_HCl__25___7_7_M_
ing17_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_009205 --> ing17_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing17_FeCl2_x_4_H2O -.-> CHEBI_86249
ing18_N2_gas["N2 gas"]
CultureMech_009205 --> ing18_N2_gas
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009205 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_009205 --> ing20_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_Calcium_pantothenate -.-> CHEBI_31345
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009205 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_009205 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_009205 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_009205 --> ing24_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing24_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing25_D____Biotin["D(+)-Biotin"]
CultureMech_009205 --> ing25_D____Biotin
CHEBI_15956["CHEBI:15956"]
ing25_D____Biotin -.-> CHEBI_15956
ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_009205 --> ing26_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing27_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_009205 --> ing27_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing27_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing28_NaOH["NaOH"]
CultureMech_009205 --> ing28_NaOH
CHEBI_32145["CHEBI:32145"]
ing28_NaOH -.-> CHEBI_32145
ing29__Na2CO3[" Na2CO3"]
CultureMech_009205 --> ing29__Na2CO3
CHEBI_29377["CHEBI:29377"]
ing29__Na2CO3 -.-> CHEBI_29377
CultureMech_009205 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3970.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 940.0, 990.0, 1000.0, 1000.0, 30.0, 10.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | HCl (25%; 7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Solution | |
| 18 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 | N2 gas | variable VARIABLE | Properties: ガス | |
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
80 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
300 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 | Vitamin B12 | 100 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
200 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
200 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 30 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 31 | FeSO4 x 7 H2O (0.2% w/v) | 10 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Solution | |
| 32 |
Methanol
CH4O 32.042 g/mol
|
0.6 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Defined component, Simple component |
| 33 |
Trimethylamine hydrochloride
C3H9N.HCl 95.573 g/mol
|
2 G_PER_L |
CHEBI:64700
trimethylamine hydrochloride
|
Role: Nutrient source; Properties: Organic compound, Defined component, Simple component |
| 34 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 940.5 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 1 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 1 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution D
Dose: 1 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Solution E
Dose: 30 G_PER_L
See mediadive_4959_Solution_E.yaml for composition
Solution F
Dose: 10 G_PER_L
See mediadive_6293_Solution_F.yaml for composition
Solution G
Dose: 10 G_PER_L
See mediadive_3625_Solution_G.yaml for composition
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.934614Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2649 |
| 2026-01-28T19:48:31.270284Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:52.096290+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:09:23.481383Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 11 duplicate ingredient(s) |
| 2026-03-13T06:20:04.018080Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009205 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.745676+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerobic Freshwater (FWM) Medium (for DSM 15978)" to "anaerobic_freshwater_fwm_medium_for_dsm_15978" for consistent matching |
| 2026-05-16T09:03:24.332447+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:48.898067+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.170332+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |