methanoculleus_bourgense_medium

CultureMech:009207 archaea COMPLEX LIQUID
Original name: Methanoculleus Bourgense Medium

Overview

External ontology
TOGO:M2652 — Methanoculleus Bourgense Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2652 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium332.pdf

Composition

flowchart LR
    CultureMech_009207["methanoculleus_bourgense_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009207 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NH4Cl["NH4Cl"]
    CultureMech_009207 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_009207 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_009207 --> ing3_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_x_9_H2O -.-> CHEBI_76209
    ing4_Na_acetate["Na-acetate"]
    CultureMech_009207 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Na2CO3["Na2CO3"]
    CultureMech_009207 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_009207 --> ing6_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing7_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD BBL)"]
    CultureMech_009207 --> ing7_Trypticase_peptone__BD_BBL_
    ing8_Na_formate["Na-formate"]
    CultureMech_009207 --> ing8_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing8_Na_formate -.-> CHEBI_62965
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
    CultureMech_009207 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_009207 --> ing10_Yeast_extract__OXOID_
    ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009207 --> ing11_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing11_Carbon_dioxide_gas -.-> CHEBI_16526
    ing12_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009207 --> ing12_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing12_Hydrogen_gas -.-> CHEBI_18276

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.2 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound
5 Na-acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Organic compound, Simple component, Defined component
6 Na2CO3
CO3.2Na 105.988 g/mol
1.5 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
8 Trypticase peptone (BD BBL) 1 G_PER_L Properties: Undefined component
9 Na-formate
CHO2.Na 68.007 g/mol
5 G_PER_L CHEBI:62965
sodium formate
 Properties: Defined component, Inorganic compound, Simple component
10 L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Organic compound, Simple component, Defined component
11 Yeast extract (OXOID) 1 G_PER_L Properties: Undefined component, Complex component
12 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
13 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Solutions

Na-resazurin solution (0.1% w/v)

Dose: 0.5 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:56.939694Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2652
2026-01-28T19:48:09.296655Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:15.017321+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:04.037222Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009207
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.765685+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanoculleus Bourgense Medium" to "methanoculleus_bourgense_medium" for consistent matching
2026-05-16T09:04:21.778306+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:31.333545+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.903996+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.