Overview
- External ontology
- TOGO:M2654 — Methanoregula (Peat) Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2654 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1280.pdf
Composition
flowchart LR
CultureMech_009209["methanoregula_peat_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009209 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_009209 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_009209 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Na_acetate["Na-acetate"]
CultureMech_009209 --> ing3_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Na_acetate -.-> CHEBI_32954
ing4_1_N_HCl["1 N HCl"]
CultureMech_009209 --> ing4_1_N_HCl
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009209 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Hydrogen_gas["Hydrogen gas"]
CultureMech_009209 --> ing6_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing6_Hydrogen_gas -.-> CHEBI_18276
ing7_HCl__25__["HCl (25%)"]
CultureMech_009209 --> ing7_HCl__25__
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009209 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009209 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009209 --> ing10_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_009209 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009209 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009209 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_ZnCl2["ZnCl2"]
CultureMech_009209 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_009209 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_009209 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_009209 --> ing17_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing17_MnSO4_x_4_H2O -.-> CHEBI_86358
ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_009209 --> ing18_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
ing19_Homo_PIPES__SIGMA_["Homo-PIPES (SIGMA)"]
CultureMech_009209 --> ing19_Homo_PIPES__SIGMA_
ing20_NaOH["NaOH"]
CultureMech_009209 --> ing20_NaOH
CHEBI_32145["CHEBI:32145"]
ing20_NaOH -.-> CHEBI_32145
ing21_N2_gas["N2 gas"]
CultureMech_009209 --> ing21_N2_gas
ing22_NaOH__0_38_M_["NaOH (0.38 M)"]
CultureMech_009209 --> ing22_NaOH__0_38_M_
ing23_Yeast_extract["Yeast extract"]
CultureMech_009209 --> ing23_Yeast_extract
ing24_2_Mercaptoethanesulfonate__coenzyme_M_["2-Mercaptoethanesulfonate (coenzyme M)"]
CultureMech_009209 --> ing24_2_Mercaptoethanesulfonate__coenzyme_M_
ing25_Biotin["Biotin"]
CultureMech_009209 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009209 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Thiamine_HCl["Thiamine-HCl"]
CultureMech_009209 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_009209 --> ing28_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing28_Pyridoxine_HCl -.-> CHEBI_30961
ing29_Folic_acid["Folic acid"]
CultureMech_009209 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_009209 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2080.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 950.0, 1000.0, 10.0, 10.0, 10.0, 100.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
14 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
27 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
17 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 5 | 1 N HCl | variable VARIABLE | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 7 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 8 | HCl (25%) | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Solution, Defined component | |
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.56 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
24 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
19 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
24 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
75 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
9 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
3.45 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
26 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.34 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | Homo-PIPES (SIGMA) | 1.58 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 21 |
NaOH
HO.Na 39.997 g/mol
|
0.18 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 22 | N2 gas | variable VARIABLE | Properties: ガス | |
| 23 | NaOH (0.38 M) | 7.2 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 24 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 25 | 2-Mercaptoethanesulfonate (coenzyme M) | 0.07 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 35 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
Solutions
Solution A
Dose: 950 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 10 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 12.55 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution D
Dose: 10 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Solution E
Dose: 10 G_PER_L
See mediadive_4959_Solution_E.yaml for composition
Solution F
Dose: 10 G_PER_L
See mediadive_6293_Solution_F.yaml for composition
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
KCl solution (0.1% w/v)
Dose: 2 G_PER_L
Role: Mineral source; Properties: Defined component, Solution
Trace elements solution
Dose: 1 G_PER_L
See mediadive_6129_Trace_elements_solution.yaml for composition
Nitrilotriacetate solution (0.5 M, neutralized with 10 N NaOH)
Dose: 4.8 G_PER_L
Role: Mineral source; Properties: Organic compound, Defined component, Simple component
TiCl3 solution (15% w/v in HCl, RIEDEL-de HAEN)
Dose: 0.55 G_PER_L
Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.946093Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2654 |
| 2026-01-28T19:48:22.543113Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1 N HCl; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:37.604220+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 11 solution(s) from ingredients |
| 2026-03-13T03:09:07.300988Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 9 duplicate ingredient(s) |
| 2026-03-13T06:20:04.073093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009209 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.799455+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanoregula (Peat) Medium" to "methanoregula_peat_medium" for consistent matching |
| 2026-05-16T09:03:14.856851+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.132177+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.950252+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:27.716903+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.494490+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |