Overview
- External ontology
- TOGO:M2668 — JCM Medium 161
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2668 Original URL: https://www.atcc.org/~/media/C09E6B9C6CF8494CAA69A2CA7DE40AB1.ashx
Composition
flowchart LR
CultureMech_009223["jcm_medium_161"]
ing0_5__Na2S___9H2O["5% Na2S . 9H2O"]
CultureMech_009223 --> ing0_5__Na2S___9H2O
ing1_NaCl["NaCl"]
CultureMech_009223 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Resazurin["Resazurin"]
CultureMech_009223 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Sulfur["Sulfur"]
CultureMech_009223 --> ing3_Sulfur
CHEBI_26833["CHEBI:26833"]
ing3_Sulfur -.-> CHEBI_26833
ing4_Peptone__Bacto__BD_211677_["Peptone, Bacto (BD 211677)"]
CultureMech_009223 --> ing4_Peptone__Bacto__BD_211677_
ing5_ATCC_Medium_1256["ATCC Medium 1256"]
CultureMech_009223 --> ing5_ATCC_Medium_1256
ing6_N2["N2"]
CultureMech_009223 --> ing6_N2
CHEBI_17997["CHEBI:17997"]
ing6_N2 -.-> CHEBI_17997
ing7_Distilled_water["Distilled water"]
CultureMech_009223 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009223 --> ing8_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4___7H2O -.-> CHEBI_31795
ing9_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009223 --> ing9_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2___2H2O -.-> CHEBI_86158
ing10_KH2PO4["KH2PO4"]
CultureMech_009223 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009223 --> ing11_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4___2H2O -.-> CHEBI_75213
ing12_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009223 --> ing12_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2___4H2O -.-> CHEBI_86368
ing13_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009223 --> ing13_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4___7H2O -.-> CHEBI_32312
ing14_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009223 --> ing14_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2___2H2O -.-> CHEBI_86318
ing15__NH4_2SO4["(NH4)2SO4"]
CultureMech_009223 --> ing15__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing15__NH4_2SO4 -.-> CHEBI_62946
ing16_FeCl3___6H2O["FeCl3 . 6H2O"]
CultureMech_009223 --> ing16_FeCl3___6H2O
CHEBI_86254["CHEBI:86254"]
ing16_FeCl3___6H2O -.-> CHEBI_86254
ing17_VOSO4___2H2O["VOSO4 . 2H2O"]
CultureMech_009223 --> ing17_VOSO4___2H2O
CHEBI_87009["CHEBI:87009"]
ing17_VOSO4___2H2O -.-> CHEBI_87009
ing18_Na2B4O7___10H2O["Na2B4O7 . 10H2O"]
CultureMech_009223 --> ing18_Na2B4O7___10H2O
CHEBI_131366["CHEBI:131366"]
ing18_Na2B4O7___10H2O -.-> CHEBI_131366
ing19_CoSO4["CoSO4"]
CultureMech_009223 --> ing19_CoSO4
CHEBI_53470["CHEBI:53470"]
ing19_CoSO4 -.-> CHEBI_53470
ing20_Yeast_Extract__BD_212750_["Yeast Extract (BD 212750)"]
CultureMech_009223 --> ing20_Yeast_Extract__BD_212750_
ing21_10_N_H2SO4["10 N H2SO4"]
CultureMech_009223 --> ing21_10_N_H2SO4
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 5% Na2S . 9H2O | 10 G_PER_L | Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution | |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 4 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Peptone, Bacto (BD 211677) | 5 G_PER_L | Properties: Undefined component | |
| 6 | ATCC Medium 1256 | 1 G_PER_L | ||
| 7 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 8 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeCl3 . 6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
VOSO4 . 2H2O
2H2O.O4S.OV 199.036 g/mol
|
0.03 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2B4O7 . 10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4.5 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CoSO4
Co.O4S 154.996 g/mol
|
0.01 G_PER_L |
CHEBI:53470
cobalt(2+) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | Yeast Extract (BD 212750) | 1 G_PER_L | Properties: Undefined component | |
| 22 | 10 N H2SO4 | variable VARIABLE | Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.009877Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2668 |
| 2026-01-28T19:48:25.258641Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: 10 N H2SO4 |
| 2026-03-13T06:20:04.359340Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009223 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.097634+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM Medium 161" to "jcm_medium_161" for consistent matching |
| 2026-05-16T09:03:38.049697+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:09.740205+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:49.644315+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.164253+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |