sulfolobus_medium_anaerobic_for_dsm_2162 (CultureMech:009226)

Overview

External ontology

TOGO:M2670 — Sulfolobus Medium (Anaerobic) (For DSM 2162)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2670 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88a.pdf

Composition

flowchart LR
    CultureMech_009226["sulfolobus_medium_anaerobic_for_dsm_2162"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009226 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_009226 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_009226 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009226 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_009226 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009226 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_009226 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_Sulfur__powder["Sulfur, powder"]
    CultureMech_009226 --> ing7_Sulfur__powder
    ing8_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_009226 --> ing8_Yeast_extract__OXOID_
    ing9_1_N_H2SO4["1 N H2SO4"]
    CultureMech_009226 --> ing9_1_N_H2SO4
    ing10_N2_gas["N2 gas"]
    CultureMech_009226 --> ing10_N2_gas
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_009226 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_009226 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_009226 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_009226 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_009226 --> ing15_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing15_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing16_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_009226 --> ing16_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing16_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing17_CoSO4["CoSO4"]
    CultureMech_009226 --> ing17_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing17_CoSO4 -.-> CHEBI_53470
    ing18_1_N_HCl["1 N HCl"]
    CultureMech_009226 --> ing18_1_N_HCl

Ingredients (19)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1000.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1000.0, 1000.0]
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.25 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.07 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.28 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
6	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.3 G_PER_L	CHEBI:62946 ammonium sulfate	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
7	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.02 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8	Sulfur, powder	10 G_PER_L		Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9	Yeast extract (OXOID)	1 G_PER_L		Properties: Undefined component, Complex component
10	1 N H2SO4	variable VARIABLE		Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
11	N2 gas	variable VARIABLE		Properties: Gas
12	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	3 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	180 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	22 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	5 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16	VOSO4 x 2 H2O 2H2O.O4S.OV 199.036 g/mol	3 G_PER_L	CHEBI:87009 vanadyl sulfate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	450 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18	CoSO4 Co.O4S 154.996 g/mol	1 G_PER_L	CHEBI:53470 cobalt(2+) sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19	1 N HCl	variable VARIABLE		Role: Solvating media; Properties: Defined component, Solution, Inorganic compound

Solutions

Allen’s trace element solution

Dose: 10 G_PER_L

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Acidianus brierleyi
Term
Id
NCBITaxon:41673
Label
Acidianus brierleyi
Scoped to variant
aerobic_brocks_salts_dsm88_sulfur_yeast_extract
Strain
DSM 3772 (also DSM 1651)
Evidence
- Reference
  doi:10.1128/mbio.01033-24
  Supports
  SUPPORT
  Explanation
  Scoped to the aerobic_brocks_salts_dsm88_sulfur_yeast_extract variant. NOT verified on the anaerobic parent (no Na2S/N2 reported); aerobic vented-bottle cultivation. | Identifiers: DSM 3772; DSM 1651
Preferred term
Metallosphaera hakonensis
Term
Id
NCBITaxon:79601
Label
Metallosphaera hakonensis
Scoped to variant
aerobic_brocks_salts_dsm88_sulfur_yeast_extract
Strain
HO1-1, DSM 7519
Genome assembly id
- GCA_003201675.2
Evidence
- Reference
  doi:10.1128/mbio.01033-24
  Supports
  SUPPORT
  Explanation
  Scoped to the aerobic_brocks_salts_dsm88_sulfur_yeast_extract variant. Differs from anaerobic parent: NH4Cl vs (NH4)2SO4, no Na2S, no N2 phase. | Identifiers: DSM 7519
Preferred term
Metallosphaera prunae
Scoped to variant
aerobic_brocks_salts_dsm88_sulfur_yeast_extract
Strain
RON 12/II, DSM 10039
Evidence
- Reference
  doi:10.1128/mbio.01033-24
  Supports
  SUPPORT
  Explanation
  Scoped to the aerobic_brocks_salts_dsm88_sulfur_yeast_extract variant. NOT the anaerobic parent formulation. | Identifiers: DSM 10039
Preferred term
Sulfurisphaera ohwakuensis
Term
Id
NCBITaxon:69656
Label
Sulfurisphaera ohwakuensis
Scoped to variant
aerobic_brocks_salts_dsm88_sulfur_yeast_extract
Strain
TA-1, DSM 12421
Evidence
- Reference
  doi:10.1128/mbio.01033-24
  Supports
  SUPPORT
  Explanation
  Scoped to the aerobic_brocks_salts_dsm88_sulfur_yeast_extract variant. Vented bottles; not anaerobic parent. | Identifiers: DSM 12421
Preferred term
Sulfurisphaera tokodaii
Term
Id
NCBITaxon:111955
Label
Sulfurisphaera tokodaii
Scoped to variant
aerobic_brocks_salts_dsm88_sulfur_yeast_extract
Strain
str. 7, DSM 16993
Evidence
- Reference
  doi:10.1128/mbio.01033-24
  Supports
  SUPPORT
  Explanation
  Scoped to the aerobic_brocks_salts_dsm88_sulfur_yeast_extract variant. NH4Cl, no Na2S, vented bottles; not the anaerobic parent. | Identifiers: DSM 16993
Preferred term
Sulfodiicoccus acidiphilus
Term
Id
NCBITaxon:1670455
Label
Sulfodiicoccus acidiphilus
Scoped to variant
reduced_anaerobic_mbsy_h2_co2
Strain
HS-1T (= JCM 31740T = InaCC Ar79T)
Genome assembly id
- GCA_003967175.1
Evidence
- Reference
  doi:10.1099/ijsem.0.001881
  Supports
  SUPPORT
  Explanation
  Closest match to the anaerobic parent of all DSM 2162 candidates (genuinely anaerobic: Na2S + reduced + H2/CO2). Basal MBS recipe is cited transitively, so exact identity is plausible but not provable from this source; scoped to the reduced_anaerobic_mbsy_h2_co2 variant. | Identifiers: JCM 31740T; InaCC Ar79T; 16S LC061218; 23S LC176656
  Snippet
  Strain HS-1T grown in modified Brock's basal salt medium with yeast extract (MBSY); anaerobic tests used MBSY reduced with Na2S (0.5 g/L) + resazurin (1 mg/L), headspace H2/CO2 (80:20, 100 kPa), optional 10 g/L elemental sulfur.

Recipe variants

Name
aerobic_brocks_salts_dsm88_sulfur_yeast_extract
Relationship
DERIVED_FROM
Description
Aerobic low-sulfate Brock's salts (DSMZ medium 88) formulation used by Willard et al. 2024 for a broad set of Sulfolobales. Distinct from the anaerobic parent in atmosphere and nitrogen source; grouped here as a derived aerobic variant.
Modifications
- Low-sulfate Brock's salts base (MgSO4 reduced/replaced); NH4Cl instead of (NH4)2SO4
- 10 g/L elemental sulfur; species-specific 0.2-1 g/L yeast extract (or sucrose + NZ-Amine)
- AEROBIC: vented serum bottles with foam stoppers; NO Na2S reducing agent; NO N2 gas phase
- 65-75C; pH 2-3 (acidified with H2SO4 or HCl)
Purpose
Aerobic sulfur-oxidation / comparative physiology cultivation of Sulfolobales (A. brierleyi, M. hakonensis, M. prunae, S. ohwakuensis, S. tokodaii).
Evidence
- Reference
  doi:10.1128/mbio.01033-24
  Supports
  SUPPORT
  Explanation
  Willard et al. 2024 is the shared source for the aerobic DSM-88 variant across these strains.
  Snippet
  All Sulfolobaceae species were grown in a low-sulfate formulation of Brock’s Salts (DSM-88 medium) containing:
Name
reduced_anaerobic_mbsy_h2_co2
Relationship
DERIVED_FROM
Description
Reduced anaerobic modified Brock's basal salt medium with yeast extract (MBSY) used by Sakai & Kurosawa 2017 for anaerobic growth tests of Sulfodiicoccus acidiphilus HS-1T. The genuinely anaerobic member of the DSM 2162 evidence set.
Modifications
- Modified Brock's basal salts + 1 g/L yeast extract (MBSY)
- Reduced with Na2S (0.5 g/L) + resazurin (1 mg/L)
- Headspace H2/CO2 (80:20) at 100 kPa; optional 10 g/L elemental sulfur
- 65-75C; pH 3.0
Purpose
Anaerobic growth testing of Sulfodiicoccus acidiphilus HS-1T.
Evidence
- Reference
  doi:10.1099/ijsem.0.001881
  Supports
  SUPPORT
  Explanation
  Sakai & Kurosawa 2017 Methods. Basal MBS recipe cited to another reference.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.016554Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2670
2026-01-28T19:48:39.397279Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: 1 N H2SO4; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: 1 N HCl
2026-03-09T08:05:06.221102+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T03:09:37.998819Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:20:04.397712Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009226
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:49.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.139234+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Sulfolobus Medium (Anaerobic) (For DSM 2162)" to "sulfolobus_medium_anaerobic_for_dsm_2162" for consistent matching
2026-05-16T09:04:32.766274+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:41:47.415545+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:33.317379+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:12.004673+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-02T06:47:50.294175+00:00	deep-research-edison-phase2	ENRICHED	Phase-2 CAUTION: this parent record is explicitly ANAEROBIC (DSMZ 88a / TOGO M2670; Na2S reducing agent + N2 gas phase). Every literature strain retrieved (Willard et al. 2024) was cultivated on AEROBIC low-sulfate Brock's salts (DSM-88) in vented serum bottles, using NH4Cl instead of (NH4)2SO4 and without Na2S/N2. These strains are therefore scoped to an aerobic Brock's-salts MediaVariant and their evidence explanations explicitly state growth is NOT verified on the anaerobic parent. S. acidocaldarius MW001, Saccharolobus solfataricus P2, and Sulfuracidifex tepidarius DSM 104736 were EXCLUDED entirely because phase-2 judged their medium a distinct record, not a variant. Sulfodiicoccus acidiphilus HS-1T is the only strain with a genuinely anaerobic (MBSY + Na2S + H2/CO2) formulation and is the closest to the parent, though its basal recipe is also cited transitively. [added 6 organism(s), 2 variant(s)]
2026-08-06T00:58:02.579053+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 6 specific strain(s) named, no community signal (#142)
2026-09-02T07:37:31.932117+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.