Overview
- External ontology
- TOGO:M2672 — Thermosphaera Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2672 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium817.pdf
Composition
flowchart LR
CultureMech_009228["thermosphaera_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009228 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009228 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NH4Cl["NH4Cl"]
CultureMech_009228 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009228 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009228 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_KCl["KCl"]
CultureMech_009228 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaHCO3["NaHCO3"]
CultureMech_009228 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Yeast_extract__BD_Bacto_["Yeast extract (BD Bacto)"]
CultureMech_009228 --> ing7_Yeast_extract__BD_Bacto_
ing8_Peptone__BD_Bacto_["Peptone (BD Bacto)"]
CultureMech_009228 --> ing8_Peptone__BD_Bacto_
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009228 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_009228 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_Biotin["Biotin"]
CultureMech_009228 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009228 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Thiamine_HCl["Thiamine-HCl"]
CultureMech_009228 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_009228 --> ing14_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_HCl -.-> CHEBI_30961
ing15_Folic_acid["Folic acid"]
CultureMech_009228 --> ing15_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing15_Folic_acid -.-> CHEBI_27470
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_009228 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_Riboflavin["Riboflavin"]
CultureMech_009228 --> ing17_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing17_Riboflavin -.-> CHEBI_17015
ing18_Nicotinic_acid["Nicotinic acid"]
CultureMech_009228 --> ing18_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing18_Nicotinic_acid -.-> CHEBI_15940
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_009228 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_D_Ca_pantothenate["D-Ca-pantothenate"]
CultureMech_009228 --> ing20_D_Ca_pantothenate
CHEBI_31345["CHEBI:31345"]
ing20_D_Ca_pantothenate -.-> CHEBI_31345
ing21_N2["N2"]
CultureMech_009228 --> ing21_N2
ing21_N2 -.-> CHEBI_17997
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1000.0, 1000.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.9 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
12 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
17 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Yeast extract (BD Bacto) | 1 G_PER_L | Properties: Undefined component | |
| 9 | Peptone (BD Bacto) | 1 G_PER_L | Properties: Undefined component | |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 21 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
| 22 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
CaCl2 x 2 H2O solution (0.1% w/v)
Dose: 7 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
K2HPO4 x 3 H2O solution (0.1% w/v)
Dose: 7 G_PER_L
Role: Buffer; Properties: Inorganic compound, Solution, Defined component
FeCl3 solution (0.01% w/v in 0.2 N HCl)
Dose: 0.5 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution
Vitamin solution
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.022259Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2672 |
| 2026-01-28T19:48:10.017969Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:16.305811+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:43.695880Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:04.422856Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009228 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.163877+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermosphaera Medium" to "thermosphaera_medium" for consistent matching |
| 2026-05-16T09:03:15.074777+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.354728+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.135192+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:49.585046+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:27.565893+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |