Overview
- External ontology
- TOGO:M2676 — Thermoproteus Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2676 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium185.pdf
Composition
flowchart LR
CultureMech_009231["thermoproteus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009231 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009231 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009231 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_009231 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Resazurin["Resazurin"]
CultureMech_009231 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009231 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009231 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_009231 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_Sulfur__powdered["Sulfur, powdered"]
CultureMech_009231 --> ing8_Sulfur__powdered
ing9_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_009231 --> ing9_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing9_CaSO4_x_2_H2O -.-> CHEBI_32583
ing10_Soluble_starch["Soluble starch"]
CultureMech_009231 --> ing10_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing10_Soluble_starch -.-> CHEBI_28017
ing11_N2_gas["N2 gas"]
CultureMech_009231 --> ing11_N2_gas
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009231 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009231 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009231 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009231 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_009231 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_NaF["NaF"]
CultureMech_009231 --> ing17_NaF
CHEBI_28741["CHEBI:28741"]
ing17_NaF -.-> CHEBI_28741
ing18_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_009231 --> ing18_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing18_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1000.0, 1000.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.492 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.014 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.556 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.264 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | Sulfur, powdered | 10 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 10 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.344 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Soluble starch | 5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 12 | N2 gas | variable VARIABLE | Properties: Gas | |
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
180 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NaF
F.Na 41.988 g/mol
|
840 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
450 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace elements solution
Dose: 1 G_PER_L
See mediadive_6129_Trace_elements_solution.yaml for composition
H2SO4 solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.033768Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2676 |
| 2026-01-28T19:48:08.839864Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: H2SO4 solution |
| 2026-03-09T08:04:14.242095+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:41.286725Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:04.474124Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009231 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.216890+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermoproteus Medium" to "thermoproteus_medium" for consistent matching |
| 2026-05-16T09:03:15.110553+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.391404+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.964963+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.600784+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:27.609878+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |