Overview
- External ontology
- TOGO:M2685 — M2GSC medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2685
Composition
flowchart LR
CultureMech_009240["m2gsc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009240 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009240 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009240 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009240 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KH2PO4["KH2PO4"]
CultureMech_009240 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_009240 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009240 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_009240 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_009240 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_CaCl2["CaCl2"]
CultureMech_009240 --> ing9_CaCl2
CHEBI_3312["CHEBI:3312"]
ing9_CaCl2 -.-> CHEBI_3312
ing10_Cellobiose["Cellobiose"]
CultureMech_009240 --> ing10_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing10_Cellobiose -.-> CHEBI_17057
ing11_Rumen_fluid["Rumen fluid"]
CultureMech_009240 --> ing11_Rumen_fluid
ing12_Glucose["Glucose"]
CultureMech_009240 --> ing12_Glucose
CHEBI_17234["CHEBI:17234"]
ing12_Glucose -.-> CHEBI_17234
ing13_Soluble_starch["Soluble starch"]
CultureMech_009240 --> ing13_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing13_Soluble_starch -.-> CHEBI_28017
ing14_Cysteine_hydrochloride["Cysteine hydrochloride"]
CultureMech_009240 --> ing14_Cysteine_hydrochloride
CHEBI_91248["CHEBI:91248"]
ing14_Cysteine_hydrochloride -.-> CHEBI_91248
ing15_Casitone["Casitone"]
CultureMech_009240 --> ing15_Casitone
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
70 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.009 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Yeast extract | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.09 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.045 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.045 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.09 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.009 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.2 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 12 | Rumen fluid | 30 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 13 |
Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 14 | Soluble starch | 0.2 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 15 |
Cysteine hydrochloride
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 16 | Casitone | 1 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.060807Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2685 |
| 2026-01-28T19:48:34.032709Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:04.599704Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009240 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.346362+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M2GSC medium" to "m2gsc_medium" for consistent matching |
| 2026-06-05T07:41:11.812669+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:51.035791+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.688152+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |