brucella_agar_plates_supplemented_with_hemin_and_vitamin_k1

CultureMech:009246 bacterial COMPLEX SOLID_AGAR
Original name: Brucella agar plates supplemented with hemin and vitamin K1

Overview

External ontology
TOGO:M2694 — Brucella agar plates supplemented with hemin and vitamin K1
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2694

Composition

flowchart LR
    CultureMech_009246["brucella_agar_plates_supplemented_with_hemin_and_vitamin_k1"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009246 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_Extract["Yeast Extract"]
    CultureMech_009246 --> ing1_Yeast_Extract
    ing2_Sodium_Chloride["Sodium Chloride"]
    CultureMech_009246 --> ing2_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing2_Sodium_Chloride -.-> CHEBI_26710
    ing3_Hemin["Hemin"]
    CultureMech_009246 --> ing3_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing3_Hemin -.-> CHEBI_50385
    ing4_Sheep_Blood__defibrinated["Sheep Blood, defibrinated"]
    CultureMech_009246 --> ing4_Sheep_Blood__defibrinated
    ing5_Glucose["Glucose"]
    CultureMech_009246 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Vitamin_K1["Vitamin K1"]
    CultureMech_009246 --> ing6_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing6_Vitamin_K1 -.-> CHEBI_18067
    ing7_Agar["Agar"]
    CultureMech_009246 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Sodium_Bisulfite["Sodium Bisulfite"]
    CultureMech_009246 --> ing8_Sodium_Bisulfite
    CHEBI_26709["CHEBI:26709"]
    ing8_Sodium_Bisulfite -.-> CHEBI_26709
    ing9_Pancreatic_Digest_of_Casein["Pancreatic Digest of Casein"]
    CultureMech_009246 --> ing9_Pancreatic_Digest_of_Casein
    ing10_Peptic_Digest_of_Animal_Tissue["Peptic Digest of Animal Tissue"]
    CultureMech_009246 --> ing10_Peptic_Digest_of_Animal_Tissue

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast Extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
3 Sodium Chloride
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
4 Hemin
C34H32ClFeN4O4 651.952 g/mol
0.005 G_PER_L CHEBI:50385
hemin
 Role: Growth factor; Properties: Defined component, Simple component, Organic compound
5 Sheep Blood, defibrinated 5 PERCENT_W_V Role: Nutrient source; Properties: Undefined component
6 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
7 Vitamin K1
C31H46O2 450.707 g/mol
0.01 G_PER_L CHEBI:18067
phylloquinone
 Role: Vitamin; Properties: Defined component, Organic compound, Simple component
8 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
9 Sodium Bisulfite
HO3S.Na 104.062 g/mol
0.1 G_PER_L CHEBI:26709
sodium hydrogensulfite
 Properties: Defined component, Inorganic compound, Simple component
10 Pancreatic Digest of Casein 10 G_PER_L Properties: Undefined component
11 Peptic Digest of Animal Tissue 10 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.070287Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2694
2026-01-28T19:48:16.049710Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:04.647190Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009246
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.396506+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Brucella agar plates supplemented with hemin and vitamin K1" to "brucella_agar_plates_supplemented_with_hemin_and_vitamin_k1" for consistent matching
2026-06-05T07:40:51.577577+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.