clostridium_thermocellum_medium_atcc_medium_1190_with_18_75_g_filter_paper_substituted_for_the_glucose (CultureMech:009249)

Overview

External ontology

TOGO:M2697 — Clostridium thermocellum medium (ATCC medium 1190) with 18.75 g filter paper substituted for the glucose

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M2697 Original URL: https://www.atcc.org/~/media/E8C2EBA220F24047A6FDE5432DA668AA.ashx

Composition

flowchart LR
    CultureMech_009249["clostridium_thermocellum_medium_atcc_medium_1190_with_18_75_g_filte..."]
    ing0_Resazurin__0_1__["Resazurin (0.1%)"]
    CultureMech_009249 --> ing0_Resazurin__0_1__
    ing1_Distilled_deionized_water["Distilled deionized water"]
    CultureMech_009249 --> ing1_Distilled_deionized_water
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009249 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009249 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2___6H2O["MgCl2 . 6H2O"]
    CultureMech_009249 --> ing4_MgCl2___6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2___6H2O -.-> CHEBI_86345
    ing5_Na2HPO4___12H2O["Na2HPO4 . 12H2O"]
    CultureMech_009249 --> ing5_Na2HPO4___12H2O
    CHEBI_34683["CHEBI:34683"]
    ing5_Na2HPO4___12H2O -.-> CHEBI_34683
    ing6_Yeast_Extract__BD_212750_["Yeast Extract (BD 212750)"]
    CultureMech_009249 --> ing6_Yeast_Extract__BD_212750_
    ing7_Whatman__1_filter_paper["Whatman #1 filter paper"]
    CultureMech_009249 --> ing7_Whatman__1_filter_paper
    ing8_Wolfe_s_Modified_Mineral_Elixir["Wolfe's Modified Mineral Elixir"]
    CultureMech_009249 --> ing8_Wolfe_s_Modified_Mineral_Elixir
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009249 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997
    ing10_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009249 --> ing10_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing10_Hydrogen_gas -.-> CHEBI_18276
    ing11_0_2_N_NaOH["0.2 N NaOH"]
    CultureMech_009249 --> ing11_0_2_N_NaOH
    ing12_Na2S___9H2O["Na2S . 9H2O"]
    CultureMech_009249 --> ing12_Na2S___9H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S___9H2O -.-> CHEBI_76209
    ing13_L_Cysteine___HCl["L-Cysteine . HCl"]
    CultureMech_009249 --> ing13_L_Cysteine___HCl
    ing14_Biotin["Biotin"]
    CultureMech_009249 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_009249 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Thiamine___HCl["Thiamine . HCl"]
    CultureMech_009249 --> ing16_Thiamine___HCl
    ing17_Pantothenic_acid_calcium_salt["Pantothenic acid calcium salt"]
    CultureMech_009249 --> ing17_Pantothenic_acid_calcium_salt
    ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_009249 --> ing18_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing19_Folic_acid["Folic acid"]
    CultureMech_009249 --> ing19_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing19_Folic_acid -.-> CHEBI_27470
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_009249 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_Riboflavin["Riboflavin"]
    CultureMech_009249 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_009249 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Thioctic_acid["Thioctic acid"]
    CultureMech_009249 --> ing23_Thioctic_acid
    CHEBI_30314["CHEBI:30314"]
    ing23_Thioctic_acid -.-> CHEBI_30314
    ing24_Distilled_water["Distilled water"]
    CultureMech_009249 --> ing24_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing24_Distilled_water -.-> CHEBI_15377
    ing25_MgSO4___7H2O["MgSO4 . 7H2O"]
    CultureMech_009249 --> ing25_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing25_MgSO4___7H2O -.-> CHEBI_31795
    ing26_NaCl["NaCl"]
    CultureMech_009249 --> ing26_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing26_NaCl -.-> CHEBI_26710
    ing27_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
    CultureMech_009249 --> ing27_Na2MoO4___2H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4___2H2O -.-> CHEBI_75213
    ing28_Boric_acid["Boric acid"]
    CultureMech_009249 --> ing28_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing28_Boric_acid -.-> CHEBI_33118
    ing29_FeSO4___7H2O["FeSO4 . 7H2O"]
    CultureMech_009249 --> ing29_FeSO4___7H2O
    CHEBI_75836["CHEBI:75836"]
    ing29_FeSO4___7H2O -.-> CHEBI_75836
    CultureMech_009249 --> more["...9 more"]

Ingredients (39)

#	Ingredient	Concentration	Ontology	Notes
1	Resazurin (0.1%)	1 G_PER_L		Role: pH dependent redox indicator; Properties: Defined component, Solution
2	Distilled deionized water	501.0 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 500.0]
3	KH2PO4 H2O4P.K 136.084 g/mol	1.5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4	NH4Cl Cl.H4N 53.492 g/mol	0.5 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5	MgCl2 . 6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.18 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6	Na2HPO4 . 12H2O HO4P.2Na 141.958 g/mol	4.2 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7	Yeast Extract (BD 212750)	2 G_PER_L		Properties: Undefined component
8	Whatman #1 filter paper	18.75 G_PER_L
9	Wolfe's Modified Mineral Elixir	5 G_PER_L
10	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
11	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Properties: ガス
12	0.2 N NaOH	200 G_PER_L		Properties: Defined component, Inorganic compound, Solution
13	Na2S . 9H2O 9H2O.2Na.S 240.182 g/mol	2.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
14	L-Cysteine . HCl	2.5 G_PER_L		Properties: Defined component, Simple component, Organic compound
15	Biotin C10H16N2O3S 244.316 g/mol	20 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	50 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17	Thiamine . HCl	5 G_PER_L		Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18	Pantothenic acid calcium salt	50 G_PER_L		Role: Vitamin; Properties: Organic compound, Defined component, Simple component
19	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	100 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
20	Folic acid C19H19N7O6 441.404 g/mol	20 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
21	Vitamin B12	1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
22	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
23	Nicotinic acid C6H5NO2 123.111 g/mol	50 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
24	Thioctic acid C8H14O2S2 206.332 g/mol	50 G_PER_L	CHEBI:30314 (R)-lipoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
25	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
26	MgSO4 . 7H2O 7H2O.Mg.O4S 246.473 g/mol	3 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27	NaCl Cl.Na 58.443 g/mol	1 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
28	Na2MoO4 . 2H2O 2H2O.MoO4.2Na 241.946 g/mol	10 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29	Boric acid H3BO3 61.833 g/mol	10 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
30	FeSO4 . 7H2O Fe.7H2O.O4S 278.013 g/mol	100 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
31	ZnSO4 . 7H2O 7H2O.O4S.Zn 287.558 g/mol	100 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
32	CuSO4 . 5H2O Cu.5H2O.O4S 249.684 g/mol	10 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
33	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	1.5 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Role: Mineral source; Properties: Organic compound, Defined component, Simple component
34	CaCl2 Ca.2Cl 110.984 g/mol	100 G_PER_L	CHEBI:3312 calcium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
35	MnSO4 . H2O H2O.Mn.O4S 169.016 g/mol	500 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
36	AlK(SO4)2 Al.K.2O4S 258.206 g/mol	10 G_PER_L	CHEBI:86463 potassium aluminium sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
37	Na2SeO3 2Na.O3Se 172.937 g/mol	1 G_PER_L	CHEBI:48843 disodium selenite	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
38	Co(NO3)2 . 6H2O Co.6H2O.2NO3 291.031 g/mol	100 G_PER_L	CHEBI:86214 cobalt dinitrate hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
39	2-3 N KOH	variable VARIABLE		Role: Mineral source; Properties: Defined component, Solution

Solutions

Vitamin Solution

Dose: 0.5 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Reducing Solution

Dose: 40 G_PER_L

See mediadive_3892_Reducing_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.077591Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2697
2026-01-28T19:48:32.385708Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: 2-3 N KOH
2026-03-09T08:04:53.769367+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T03:09:25.243619Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 3 duplicate ingredient(s)
2026-03-13T06:20:04.686545Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009249
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:30:34.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.436561+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Clostridium thermocellum medium (ATCC medium 1190) with 18.75 g filter paper substituted for the glucose" to "clostridium_thermocellum_medium_atcc_medium_1190_with_18_75_g_filter_paper_substituted_for_the_glucose" for consistent matching
2026-05-16T09:03:27.965782+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:54.344312+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:39.708983+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:54.853210+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.