Overview
- External ontology
- TOGO:M2699 — Modified Schaedler medium (Infect. Immun. 29:768-777,1980) without glucose but supplemented with 0.1 M sodium lactate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2699 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1669.pdf
Composition
flowchart LR
CultureMech_009251["modified_schaedler_medium_infect_immun_29_768_777_1980_without_gluc..."]
ing0_Distilled_water["Distilled water"]
CultureMech_009251 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009251 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009251 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_009251 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_009251 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Sodium_lactate["Sodium lactate"]
CultureMech_009251 --> ing5_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing5_Sodium_lactate -.-> CHEBI_75228
ing6_Tris__hydroxymethyl_aminomethane__3_00_g["Tris (hydroxymethyl aminomethane) 3.00 g"]
CultureMech_009251 --> ing6_Tris__hydroxymethyl_aminomethane__3_00_g
ing7_Hemin["Hemin"]
CultureMech_009251 --> ing7_Hemin
CHEBI_50385["CHEBI:50385"]
ing7_Hemin -.-> CHEBI_50385
ing8_Peptone_mixture["Peptone mixture"]
CultureMech_009251 --> ing8_Peptone_mixture
ing9_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_009251 --> ing9_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Casein_peptone["Casein peptone"]
CultureMech_009251 --> ing10_Casein_peptone
ing11_Soy_peptone["Soy peptone"]
CultureMech_009251 --> ing11_Soy_peptone
ing12_CO2_["CO2 "]
CultureMech_009251 --> ing12_CO2_
CHEBI_16526["CHEBI:16526"]
ing12_CO2_ -.-> CHEBI_16526
ing13_N2["N2"]
CultureMech_009251 --> ing13_N2
CHEBI_17997["CHEBI:17997"]
ing13_N2 -.-> CHEBI_17997
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1.66 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.83 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 6 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.1 MOLAR |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 7 | Tris (hydroxymethyl aminomethane) 3.00 g | 3 G_PER_L | Role: Buffer; Properties: Organic compound, Simple component, Defined component | |
| 8 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
0.01 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Simple component, Organic compound, Defined component |
| 9 | Peptone mixture | 5 G_PER_L | Properties: Undefined component | |
| 10 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.4 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 11 | Casein peptone | 5.66 G_PER_L | Properties: Undefined component | |
| 12 | Soy peptone | 1 G_PER_L | Properties: Undefined component | |
| 13 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 14 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.082799Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2699 |
| 2026-01-28T19:48:11.142816Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 ; Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:04.700383Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009251 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.451556+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Schaedler medium (Infect. Immun. 29:768-777,1980) without glucose but supplemented with 0.1 M sodium lactate" to "modified_schaedler_medium_infect_immun_29_768_777_1980_without_glucose_but_supplemented_with_0_1_m_sodium_lactate" for consistent matching |
| 2026-05-16T09:04:26.212379+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:37.716728+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.934182+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |