Overview
- External ontology
- TOGO:M2701 — Clostridium Cellulolytic medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2701 Original URL: https://www.atcc.org/~/media/E4C9EF983C184615BD6DB87D147DE3AA.ashx
Composition
flowchart LR
CultureMech_009254["clostridium_cellulolytic_medium"]
ing0_Yeast_Extract["Yeast Extract"]
CultureMech_009254 --> ing0_Yeast_Extract
ing1_CaCl2_x_2H2O["CaCl2 x 2H2O"]
CultureMech_009254 --> ing1_CaCl2_x_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_009254 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Resazurin["Resazurin"]
CultureMech_009254 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_MgCl2_x_6H2O["MgCl2 x 6H2O"]
CultureMech_009254 --> ing4_MgCl2_x_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6H2O -.-> CHEBI_86345
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_009254 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_K2HPO4_x_3H2O["K2HPO4 x 3H2O"]
CultureMech_009254 --> ing6_K2HPO4_x_3H2O
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4_x_3H2O -.-> CHEBI_131527
ing7_Cellulose["Cellulose"]
CultureMech_009254 --> ing7_Cellulose
CHEBI_18246["CHEBI:18246"]
ing7_Cellulose -.-> CHEBI_18246
ing8_FeSO4["FeSO4"]
CultureMech_009254 --> ing8_FeSO4
CHEBI_75832["CHEBI:75832"]
ing8_FeSO4 -.-> CHEBI_75832
ing9_DI_Water["DI Water"]
CultureMech_009254 --> ing9_DI_Water
ing10_Cysteine_HCl["Cysteine HCl"]
CultureMech_009254 --> ing10_Cysteine_HCl
ing11_Nitrogen["Nitrogen"]
CultureMech_009254 --> ing11_Nitrogen
CHEBI_17997["CHEBI:17997"]
ing11_Nitrogen -.-> CHEBI_17997
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast Extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
CaCl2 x 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
2 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 5 |
MgCl2 x 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4 x 3H2O
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
7.5 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 9 |
FeSO4
Fe.O4S 151.908 g/mol
|
1.25 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 10 | DI Water | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound | |
| 11 | Cysteine HCl | 1 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 12 |
Nitrogen
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
NaOH solution
Dose: variable VARIABLE
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.084663Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2701 |
| 2026-01-28T19:48:17.954989Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH solution; Added default concentration for ingredient: Nitrogen |
| 2026-03-09T08:04:29.732158+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:04.716416Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009254 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.469437+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Clostridium Cellulolytic medium" to "clostridium_cellulolytic_medium" for consistent matching |
| 2026-05-16T09:03:27.651392+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:53.820484+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.660387+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |