Overview
- External ontology
- TOGO:M2707 — Methanobacteria medium (ATCC medium 1045) with 0.2% yeast extract and 0.5% glucose
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2707 Original URL: https://www.atcc.org/~/media/A5AC7370FB084A36A7CA2B95EA358AD9.ashx
Composition
flowchart LR
CultureMech_009258["methanobacteria_medium_atcc_medium_1045_with_0_2_yeast_extract_and_..."]
ing0_Distilled_water["Distilled water"]
CultureMech_009258 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Agar["Agar"]
CultureMech_009258 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Cysteine_Sulfide_Reducing_Agent["Cysteine-Sulfide Reducing Agent"]
CultureMech_009258 --> ing2_Cysteine_Sulfide_Reducing_Agent
ing3_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009258 --> ing3_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing3_Carbon_dioxide_gas -.-> CHEBI_16526
ing4_Nitrogen_gas["Nitrogen gas"]
CultureMech_009258 --> ing4_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing4_Nitrogen_gas -.-> CHEBI_17997
ing5_K2HPO4["K2HPO4"]
CultureMech_009258 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009258 --> ing6_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4___7H2O -.-> CHEBI_31795
ing7_NaCl["NaCl"]
CultureMech_009258 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009258 --> ing8_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2___2H2O -.-> CHEBI_86158
ing9_KH2PO4["KH2PO4"]
CultureMech_009258 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_009258 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_Na2CO3["Na2CO3"]
CultureMech_009258 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009258 --> ing12_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4___2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_009258 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009258 --> ing14_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4___7H2O -.-> CHEBI_75836
ing15_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009258 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009258 --> ing16_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4___7H2O -.-> CHEBI_32312
ing17_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009258 --> ing17_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4___5H2O -.-> CHEBI_31440
ing18_AlK_SO4_2___12H2O["AlK(SO4)2 . 12H2O"]
CultureMech_009258 --> ing18_AlK_SO4_2___12H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2___12H2O -.-> CHEBI_86465
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009258 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_CaCl2["CaCl2"]
CultureMech_009258 --> ing20_CaCl2
CHEBI_3312["CHEBI:3312"]
ing20_CaCl2 -.-> CHEBI_3312
ing21_MnSO4___H2O["MnSO4 . H2O"]
CultureMech_009258 --> ing21_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4___H2O -.-> CHEBI_86364
ing22_Biotin["Biotin"]
CultureMech_009258 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009258 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Thiamine___HCl["Thiamine . HCl"]
CultureMech_009258 --> ing24_Thiamine___HCl
ing25_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_009258 --> ing25_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_D_____pantothenate -.-> CHEBI_31345
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009258 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_009258 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_009258 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_Riboflavin["Riboflavin"]
CultureMech_009258 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_009258 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
959.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 8 duplicates: 835.0, 1.0, 1.0, 100.0, 1.0, 1.0, 10.0, 10.0] |
| 2 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 3 | Cysteine-Sulfide Reducing Agent | 20 G_PER_L | ||
| 4 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 5 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.4, 3.0] |
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
13.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 12.0, 1.0] |
| 9 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
8 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
AlK(SO4)2 . 12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 21 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 | Thiamine . HCl | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 26 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 33 | L-Cysteine . HCl | 300 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 34 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
300 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Resazurin (0.025% solution)
Dose: 4 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Mineral Solution 2
Dose: 50 G_PER_L
See mediadive_4674_Mineral_solution_2.yaml for composition
Mineral Solution 1
Dose: 25 G_PER_L
See mediadive_883_Mineral_solution_1.yaml for composition
Sodium Carbonate Solution
Dose: 50 G_PER_L
Wolfe's Mineral Solution
Dose: 10 G_PER_L
See mediadive_3952_Wolfe_s_mineral_solution.yaml for composition
Wolfe's Vitamin Solution
Dose: 10 G_PER_L
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
L-Cysteine . HCl solution
Dose: 10 G_PER_L
Na2S . 9H2O solution
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.093536Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2707 |
| 2026-01-28T19:48:09.519635Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: KOH solution; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-02-06T05:10:15.184121Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:15.402369+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 9 solution(s) from ingredients |
| 2026-03-13T03:08:42.611199Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 12 duplicate ingredient(s) |
| 2026-03-13T06:20:04.763962Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009258 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.522664+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanobacteria medium (ATCC medium 1045) with 0.2% yeast extract and 0.5% glucose" to "methanobacteria_medium_atcc_medium_1045_with_0_2_yeast_extract_and_0_5_glucose" for consistent matching |
| 2026-05-16T09:04:21.279169+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:30.584625+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:22.107644+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:06.610515+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |