chopped_meat_medium (CultureMech:009262)

Overview

External ontology

TOGO:M2710 — Chopped meat medium

Applications

Microbial cultivation

pH

7.0

Notes

TOGO M2710 imports ATCC Medium 593 Chopped meat medium. ATCC 593 lists 500.0 g fat-free ground beef, 1.0 L distilled water, 25.0 ml N NaOH, 30.0 g peptone, 5.0 g yeast extract, 5.0 g K2HPO4, 4.0 ml 0.025% resazurin solution, and 0.5 g L-cysteine HCl, adjusts the medium to pH 7.0, and dispenses it under 97% nitrogen / 3% hydrogen.

Composition

flowchart LR
    CultureMech_009262["chopped_meat_medium"]
    ing0_Ground_beef__free_of_fat_["Ground beef (free of fat)"]
    CultureMech_009262 --> ing0_Ground_beef__free_of_fat_
    ing1_Distilled_water["Distilled water"]
    CultureMech_009262 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Peptone["Peptone"]
    CultureMech_009262 --> ing2_Peptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_009262 --> ing3_Yeast_extract
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009262 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_L_cysteine___HCl["L-cysteine . HCl"]
    CultureMech_009262 --> ing5_L_cysteine___HCl
    CHEBI_91247["CHEBI:91247"]
    ing5_L_cysteine___HCl -.-> CHEBI_91247
    ing6_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009262 --> ing6_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing6_Nitrogen_gas -.-> CHEBI_17997
    ing7_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009262 --> ing7_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing7_Hydrogen_gas -.-> CHEBI_18276

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	Ground beef (free of fat)	500.0 G_PER_L		ATCC Medium 593 lists 500.0 g/L fat-free ground beef.
2	Distilled water H2O 18.015 g/mol	1000.0 ML_PER_L	CHEBI:15377 water	ATCC Medium 593 starts with 1.0 L distilled water and restores the filtrate to a final 1.0 L volume.
3	Peptone	30.0 G_PER_L	MICRO:0000178 (source link unavailable) peptone	ATCC Medium 593 lists 30.0 g/L peptone.
4	Yeast extract	5.0 G_PER_L	FOODON:03315426 yeast extract	ATCC Medium 593 lists 5.0 g/L yeast extract.
5	K2HPO4 HO4P.2K 174.174 g/mol	5.0 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	ATCC Medium 593 lists 5.0 g/L K2HPO4.
6	L-cysteine . HCl C3H7NO2S.HCl 157.622 g/mol	0.5 G_PER_L	CHEBI:91247 L-cysteine hydrochloride	ATCC Medium 593 adds 0.5 g/L L-cysteine HCl.
7	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	ATCC Medium 593 uses 97% nitrogen / 3% hydrogen while dispensing.
8	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	ATCC Medium 593 uses 97% nitrogen / 3% hydrogen while dispensing.

Preparation

Use lean beef or horse meat; remove fat and connective tissue before grinding. Mix meat, distilled water, and N NaOH and bring to a boil while stirring.
Cool to room temperature, skim fat off the surface, and filter, retaining both meat particles and filtrate. Add enough distilled water to restore 1.0 L of filtrate.
Add peptone, yeast extract, K2HPO4, any glucose supplement, and 0.025% resazurin solution to the filtrate, then boil and cool.
Add L-cysteine HCl and adjust to pH 7.0.
Under 97% nitrogen / 3% hydrogen, dispense 7 ml medium over one part meat particles to four to five parts fluid per tube. Cap with butyl rubber stoppers under N2/H2 and autoclave for 15 minutes under fast exhaust.

Solutions

N NaOH

Dose: 25.0 ML_PER_L

ATCC Medium 593 lists 25.0 ml/L N NaOH for the boiled meat extraction.

0.025% Resazurin solution

Dose: 4.0 ML_PER_L

ATCC Medium 593 adds 4.0 ml/L 0.025% resazurin solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:57.105993Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2710
2026-01-28T19:48:25.477642Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:42.183888+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 1 solution(s) from ingredients
2026-03-13T06:20:04.810290Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009262
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name
2026-03-17T06:30:19.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.571606+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Chopped meat medium" to "chopped_meat_medium" for consistent matching
2026-05-16T09:03:15.329872+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:38.613544+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.683089+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00	repair_togo_m2710_m2754_atcc_chopped_meat_score15.py	RESOLVED_TOGO_ATCC_CHOPPED_MEAT_SCORE15	TOGO M2710 imports ATCC Medium 593 Chopped meat medium. ATCC 593 lists 500.0 g fat-free ground beef, 1.0 L distilled water, 25.0 ml N NaOH, 30.0 g peptone, 5.0 g yeast extract, 5.0 g K2HPO4, 4.0 ml 0.025% resazurin solution, and 0.5 g L-cysteine HCl, adjusts the medium to pH 7.0, and dispenses it under 97% nitrogen / 3% hydrogen. Corrected imported water, N NaOH, and 0.025% resazurin volumes to ml/L units; retained the anaerobic N2/H2 headspace gases as variable gas-phase ingredients; grounded the disclosed chemical and extract ingredients; and left fat-free ground beef intentionally unmapped.
2026-09-11T00:00:00-07:00	repair_togo_m2710_m2754_atcc_chopped_meat_score15.py	LINKED_M2754_TO_M2710_SUPPLEMENTED_VARIANT	Linked ATCC Medium 735 as a glucose-supplemented ATCC Medium 593 variant.