Overview
- External ontology
- TOGO:M2721 — Chopped Meat Carbohydrate Medium w/Tween 80
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2721 Original URL: https://www.atcc.org/~/media/D1EF22ABE8034FE5B83EFF9D1BC65E18.ashx
Composition
flowchart LR
CultureMech_009272["chopped_meat_carbohydrate_medium_w_tween_80"]
ing0_NaOH__1N_["NaOH (1N)"]
CultureMech_009272 --> ing0_NaOH__1N_
ing1_DI_Water["DI Water"]
CultureMech_009272 --> ing1_DI_Water
ing2_Ground_Beef__fat_free_["Ground Beef (fat-free)"]
CultureMech_009272 --> ing2_Ground_Beef__fat_free_
ing3_Resazurin_Soln__0_025__["Resazurin Soln (0.025%)"]
CultureMech_009272 --> ing3_Resazurin_Soln__0_025__
ing4_Yeast_Extract["Yeast Extract"]
CultureMech_009272 --> ing4_Yeast_Extract
ing5_K2HPO4["K2HPO4"]
CultureMech_009272 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Starch["Starch"]
CultureMech_009272 --> ing6_Starch
CHEBI_28017["CHEBI:28017"]
ing6_Starch -.-> CHEBI_28017
ing7_Tween_80["Tween 80"]
CultureMech_009272 --> ing7_Tween_80
CHEBI_53426["CHEBI:53426"]
ing7_Tween_80 -.-> CHEBI_53426
ing8_Cellobiose["Cellobiose"]
CultureMech_009272 --> ing8_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing8_Cellobiose -.-> CHEBI_17057
ing9_Maltose["Maltose"]
CultureMech_009272 --> ing9_Maltose
CHEBI_18167["CHEBI:18167"]
ing9_Maltose -.-> CHEBI_18167
ing10_Glucose["Glucose"]
CultureMech_009272 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_Agar["Agar"]
CultureMech_009272 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
ing12_Peptone["Peptone"]
CultureMech_009272 --> ing12_Peptone
ing13_Cysteine["Cysteine"]
CultureMech_009272 --> ing13_Cysteine
CHEBI_15356["CHEBI:15356"]
ing13_Cysteine -.-> CHEBI_15356
ing14_Nitrogen_gas["Nitrogen gas"]
CultureMech_009272 --> ing14_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing14_Nitrogen_gas -.-> CHEBI_17997
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | NaOH (1N) | 25 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 2 | DI Water | 1000 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component | |
| 3 | Ground Beef (fat-free) | 500 G_PER_L | Properties: Undefined component | |
| 4 | Resazurin Soln (0.025%) | 4 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 5 | Yeast Extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Defined component, Organic compound, Complex component |
| 8 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 9 |
Cellobiose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 |
Maltose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 12 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 13 | Peptone | 30 G_PER_L | ||
| 14 |
Cysteine
C3H7NO2S 121.161 g/mol
|
0.5 G_PER_L |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 15 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.138582Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2721 |
| 2026-01-28T19:48:25.108051Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas |
| 2026-03-13T06:20:04.958000Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009272 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.726470+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chopped Meat Carbohydrate Medium w/Tween 80" to "chopped_meat_carbohydrate_medium_w_tween_80" for consistent matching |
| 2026-05-16T09:03:27.349143+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:53.397312+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.501781+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |