Overview
- External ontology
- TOGO:M2726 — Clostridium medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2726 Original URL: https://www.atcc.org/~/media/23CECE37C3794402A34CFDF21CA2DC37.ashx
Composition
flowchart LR
CultureMech_009276["clostridium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009276 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Peptone["Peptone"]
CultureMech_009276 --> ing1_Peptone
ing2_K2HPO4["K2HPO4"]
CultureMech_009276 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaHCO3["NaHCO3"]
CultureMech_009276 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Fructose["Fructose"]
CultureMech_009276 --> ing4_Fructose
CHEBI_28645["CHEBI:28645"]
ing4_Fructose -.-> CHEBI_28645
ing5_Sodium_thioglycollate["Sodium thioglycollate"]
CultureMech_009276 --> ing5_Sodium_thioglycollate
CHEBI_86481["CHEBI:86481"]
ing5_Sodium_thioglycollate -.-> CHEBI_86481
ing6_CO2["CO2"]
CultureMech_009276 --> ing6_CO2
CHEBI_16526["CHEBI:16526"]
ing6_CO2 -.-> CHEBI_16526
ing7_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009276 --> ing7_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4___2H2O -.-> CHEBI_75213
ing8_H3BO3["H3BO3"]
CultureMech_009276 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009276 --> ing9_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4___7H2O -.-> CHEBI_75836
ing10_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009276 --> ing10_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2___4H2O -.-> CHEBI_86368
ing11_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009276 --> ing11_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2___6H2O -.-> CHEBI_35696
ing12_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009276 --> ing12_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2___6H2O -.-> CHEBI_34887
ing13_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009276 --> ing13_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4___7H2O -.-> CHEBI_32312
ing14_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009276 --> ing14_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2___2H2O -.-> CHEBI_86318
ing15_EDTA["EDTA"]
CultureMech_009276 --> ing15_EDTA
CHEBI_4735["CHEBI:4735"]
ing15_EDTA -.-> CHEBI_4735
ing16_Biotin["Biotin"]
CultureMech_009276 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009276 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_009276 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_009276 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Thiamine["Thiamine"]
CultureMech_009276 --> ing20_Thiamine
CHEBI_18385["CHEBI:18385"]
ing20_Thiamine -.-> CHEBI_18385
ing21_Pantothenic_acid["Pantothenic acid"]
CultureMech_009276 --> ing21_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing21_Pantothenic_acid -.-> CHEBI_7916
ing22_Pyridoxine["Pyridoxine"]
CultureMech_009276 --> ing22_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing22_Pyridoxine -.-> CHEBI_16709
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1077.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 600.0, 376.0, 1.0, 100.0] |
| 2 | Peptone | 5 G_PER_L | ||
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
20 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Fructose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
Sodium thioglycollate
C2H3O2S.Na 114.101 g/mol
|
0.75 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Organic compound, Simple component |
| 7 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 8 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
3 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.02 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
1 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 | Vitamin B12 | 2 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.1 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.025 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
0.05 G_PER_L |
CHEBI:16709
pyridoxine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution 1
Dose: 600 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Solution 2
Dose: 400 G_PER_L
See mediadive_6109_Solution_2.yaml for composition
Trace Element Solution
Dose: 10 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Vitamin Solution
Dose: 14 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.152640Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2726 |
| 2026-01-28T19:48:41.739071Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-09T08:05:10.395253+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:42.170475Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:05.022712Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009276 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.795676+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Clostridium medium" to "clostridium_medium" for consistent matching |
| 2026-05-16T09:03:15.472189+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.754955+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.736733+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |