Overview
- External ontology
- TOGO:M2727 — Anaerobic cellulolytic medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2727 Original URL: https://www.atcc.org/~/media/AA128C35BF4F4890A963D7A2162315D2.ashx
Composition
flowchart LR
CultureMech_009277["anaerobic_cellulolytic_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009277 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009277 --> ing1_Yeast_extract
ing2_NH4Cl["NH4Cl"]
CultureMech_009277 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_K2HPO4["K2HPO4"]
CultureMech_009277 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_009277 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_KCl["KCl"]
CultureMech_009277 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_MgSO4["MgSO4"]
CultureMech_009277 --> ing6_MgSO4
CHEBI_32599["CHEBI:32599"]
ing6_MgSO4 -.-> CHEBI_32599
ing7_Cellobiose["Cellobiose"]
CultureMech_009277 --> ing7_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing7_Cellobiose -.-> CHEBI_17057
ing8_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009277 --> ing8_L_Cysteine___HCl
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009277 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Nitrogen_gas["Nitrogen gas"]
CultureMech_009277 --> ing10_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing10_Nitrogen_gas -.-> CHEBI_17997
ing11_Hydrogen_gas["Hydrogen gas"]
CultureMech_009277 --> ing11_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing11_Hydrogen_gas -.-> CHEBI_18276
ing12_Na2S___9H2O["Na2S . 9H2O"]
CultureMech_009277 --> ing12_Na2S___9H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S___9H2O -.-> CHEBI_76209
ing13_Na2CO3["Na2CO3"]
CultureMech_009277 --> ing13_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing13_Na2CO3 -.-> CHEBI_29377
ing14_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009277 --> ing14_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4___7H2O -.-> CHEBI_31795
ing15_NaCl["NaCl"]
CultureMech_009277 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
ing16_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009277 --> ing16_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4___2H2O -.-> CHEBI_75213
ing17_H3BO3["H3BO3"]
CultureMech_009277 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009277 --> ing18_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4___7H2O -.-> CHEBI_75836
ing19_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009277 --> ing19_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing19_CoCl2___6H2O -.-> CHEBI_35696
ing20_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009277 --> ing20_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4___7H2O -.-> CHEBI_32312
ing21_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009277 --> ing21_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4___5H2O -.-> CHEBI_31440
ing22_AlK_SO4_2___12H2O["AlK(SO4)2 . 12H2O"]
CultureMech_009277 --> ing22_AlK_SO4_2___12H2O
CHEBI_86465["CHEBI:86465"]
ing22_AlK_SO4_2___12H2O -.-> CHEBI_86465
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009277 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_CaCl2["CaCl2"]
CultureMech_009277 --> ing24_CaCl2
CHEBI_3312["CHEBI:3312"]
ing24_CaCl2 -.-> CHEBI_3312
ing25_MnSO4___H2O["MnSO4 . H2O"]
CultureMech_009277 --> ing25_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing25_MnSO4___H2O -.-> CHEBI_86364
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.5 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Cellobiose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 9 | L-Cysteine . HCl | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 13 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
0.15 PERCENT_W_V |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.1 PERCENT_W_V |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
AlK(SO4)2 . 12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 25 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Wolfe's Mineral Solution
Dose: 20 G_PER_L
See mediadive_3952_Wolfe_s_mineral_solution.yaml for composition
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.156077Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2727 |
| 2026-01-28T19:48:36.563096Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:05:00.961886+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:32.808613Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:05.037034Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009277 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.810135+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerobic cellulolytic medium" to "anaerobic_cellulolytic_medium" for consistent matching |
| 2026-05-16T09:03:24.300608+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:48.860586+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:36.206745+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:53.157218+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:56.317910+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |