thermococcus_medium

CultureMech:009290 archaea COMPLEX LIQUID
Original name: Thermococcus Medium

Overview

External ontology
TOGO:M273 — Thermococcus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M273 Original source: JCM - JCM_M280 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=280

Composition

flowchart LR
    CultureMech_009290["thermococcus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009290 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009290 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009290 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009290 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009290 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_009290 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009290 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009290 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_009290 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_KCl["KCl"]
    CultureMech_009290 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_009290 --> ing10_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing10_Sulfur__powder_ -.-> CHEBI_33403
    ing11_NaBr["NaBr"]
    CultureMech_009290 --> ing11_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing11_NaBr -.-> CHEBI_63004
    ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009290 --> ing12_Yeast_extract__BD_Difco_
    ing13_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_009290 --> ing13_Tryptone__BD_Difco_
    ing14_N2["N2"]
    CultureMech_009290 --> ing14_N2
    CHEBI_17997["CHEBI:17997"]
    ing14_N2 -.-> CHEBI_17997

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
3.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
5 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
2.8 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Simple component, Defined component
9 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
0.025 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 Sulfur (powder) 10 G_PER_L CHEBI:33403
elemental sulfur
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
12 NaBr
Br.Na 102.894 g/mol
10 G_PER_L CHEBI:63004
sodium bromide
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
13 Yeast extract (BD-Difco) 3 G_PER_L Properties: Undefined component
14 Tryptone (BD-Difco) 3 G_PER_L Properties: Undefined component
15 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T06:35:54.740865Z togo-import Imported from TOGO Medium Source: JCM, ID: M273
2026-01-28T19:48:24.620388Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:40.714315+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:05.269707Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009290
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.164952+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Thermococcus Medium" to "thermococcus_medium" for consistent matching
2026-05-16T09:03:15.702658+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:38.980355+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:28.489918+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:49.829345+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-10T00:00:00-07:00 repair_archaea_ph_wrappers_score20.py LINKED_ARCHAEA_PH_WRAPPER_SCORE20_CHILDREN Added or refreshed reciprocal pH-variant link for archaea/TOGO_M345_Thermococcus_Medium_pH_6.0.yaml.