Overview
- External ontology
- TOGO:M2741 — Clostridium (GS) Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2741 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium255.pdf
Composition
flowchart LR
CultureMech_009292["clostridium_gs_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009292 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009292 --> ing1_Yeast_extract
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009292 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009292 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009292 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_009292 --> ing5_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing6_Cellobiose["Cellobiose"]
CultureMech_009292 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_Morpholinopropane_sulfonic_acid_["Morpholinopropane sulfonic acid "]
CultureMech_009292 --> ing7_Morpholinopropane_sulfonic_acid_
CHEBI_44115["CHEBI:44115"]
ing7_Morpholinopropane_sulfonic_acid_ -.-> CHEBI_44115
ing8_Urea["Urea"]
CultureMech_009292 --> ing8_Urea
CHEBI_16199["CHEBI:16199"]
ing8_Urea -.-> CHEBI_16199
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
CultureMech_009292 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_N2_gas["N2 gas"]
CultureMech_009292 --> ing10_N2_gas
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 6 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 8 |
Morpholinopropane sulfonic acid
C7H15NO4S 209.267 g/mol
|
10 G_PER_L |
CHEBI:44115
3-(N-morpholino)propanesulfonic acid
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 9 |
Urea
CH4N2O 60.056 g/mol
|
2 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Organic compound, Simple component |
| 10 |
L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 11 | N2 gas | variable VARIABLE | Properties: Gas |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
FeSO4 x 7 H2O solution (0.1% w/v in 0.1 N H2SO4)
Dose: 1.25 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.208140Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2741 |
| 2026-01-28T19:48:32.834448Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:54.333936+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:05.285864Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009292 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.182668+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Clostridium (GS) Medium" to "clostridium_gs_medium" for consistent matching |
| 2026-05-16T09:03:15.721370+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.998792+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.833913+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |