Overview
- External ontology
- TOGO:M2745 — Dehalobacter Restrictus Medium (TCE)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2745 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium732.pdf
Composition
flowchart LR
CultureMech_009296["dehalobacter_restrictus_medium_tce"]
ing0_Distilled_water["Distilled water"]
CultureMech_009296 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_K2HPO4["K2HPO4"]
CultureMech_009296 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Na_acetate["Na-acetate"]
CultureMech_009296 --> ing2_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Na_acetate -.-> CHEBI_32954
ing3_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
CultureMech_009296 --> ing3_NaH2PO4_x_H2O
CHEBI_37585["CHEBI:37585"]
ing3_NaH2PO4_x_H2O -.-> CHEBI_37585
ing4_Peptone__BD_Bacto_["Peptone (BD Bacto)"]
CultureMech_009296 --> ing4_Peptone__BD_Bacto_
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009296 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Nitrogen_gas["Nitrogen gas"]
CultureMech_009296 --> ing6_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing6_Nitrogen_gas -.-> CHEBI_17997
ing7_NaHCO3["NaHCO3"]
CultureMech_009296 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8__NH4_2CO3["(NH4)2CO3"]
CultureMech_009296 --> ing8__NH4_2CO3
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009296 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009296 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_N2_gas["N2 gas"]
CultureMech_009296 --> ing11_N2_gas
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009296 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_009296 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009296 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009296 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009296 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_009296 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_009296 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_AlCl3["AlCl3"]
CultureMech_009296 --> ing19_AlCl3
CHEBI_30114["CHEBI:30114"]
ing19_AlCl3 -.-> CHEBI_30114
ing20_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_009296 --> ing20_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2_x_4_H2O -.-> CHEBI_86249
ing21_Na2_EDTA["Na2-EDTA"]
CultureMech_009296 --> ing21_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na2_EDTA -.-> CHEBI_64734
ing22_2N_NaOH["2N NaOH"]
CultureMech_009296 --> ing22_2N_NaOH
ing23_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009296 --> ing23_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing23_Na2S_x_9_H2O -.-> CHEBI_76209
ing24_Hexadecane["Hexadecane"]
CultureMech_009296 --> ing24_Hexadecane
CHEBI_45296["CHEBI:45296"]
ing24_Hexadecane -.-> CHEBI_45296
ing25_Tetrachloroethene["Tetrachloroethene"]
CultureMech_009296 --> ing25_Tetrachloroethene
CHEBI_17300["CHEBI:17300"]
ing25_Tetrachloroethene -.-> CHEBI_17300
ing26_Biotin["Biotin"]
CultureMech_009296 --> ing26_Biotin
CHEBI_15956["CHEBI:15956"]
ing26_Biotin -.-> CHEBI_15956
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009296 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Thiamine_HCl["Thiamine-HCl"]
CultureMech_009296 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_009296 --> ing29_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing29_Pyridoxine_HCl -.-> CHEBI_30961
CultureMech_009296 --> more["...10 more"]
Ingredients (40)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3980.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 860.0, 100.0, 10.0, 1000.0, 10.0, 1000.0, 1000.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.65 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.46 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 4 |
NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
|
0.17 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Peptone (BD Bacto) | 0.1 G_PER_L | Properties: Undefined component | |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 7 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.73 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 | (NH4)2CO3 | 0.27 G_PER_L | Role: Nitrogen source | |
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.11 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | N2 gas | variable VARIABLE | Properties: Gas | |
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
AlCl3
AlCl3 133.341 g/mol
|
10 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 23 | 2N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 24 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Hexadecane
C16H34 226.448 g/mol
|
13.5 G_PER_L |
CHEBI:45296
hexadecane
|
Role: Carbon source |
| 26 |
Tetrachloroethene
C2Cl4 165.834 g/mol
|
1.5 G_PER_L |
CHEBI:17300
tetrachloroethene
|
|
| 27 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
85.0 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 5.0, 80.0] |
| 29 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 | Vitamin B12 | 100.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 0.1, 100.0] |
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
205.0 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 5.0, 200.0] |
| 35 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 36 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
| 37 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
300 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 39 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
200 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 40 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 860 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 10 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution D
Dose: 1 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Solution E
Dose: 10 G_PER_L
See mediadive_4959_Solution_E.yaml for composition
Solution F
Dose: 10 G_PER_L
See mediadive_6293_Solution_F.yaml for composition
Solution G
Dose: 15 G_PER_L
See mediadive_3625_Solution_G.yaml for composition
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Vitamin solution of medium 141
Dose: 9 G_PER_L
Vitamin solution of medium 503
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.215954Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2745 |
| 2026-01-28T19:48:33.747010Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: 2N NaOH; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:55.965415+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 10 solution(s) from ingredients |
| 2026-03-13T03:09:27.511993Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 17 duplicate ingredient(s) |
| 2026-03-13T06:20:05.332859Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009296 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.232099+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Dehalobacter Restrictus Medium (TCE)" to "dehalobacter_restrictus_medium_tce" for consistent matching |
| 2026-05-16T09:03:15.772762+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.050547+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.561731+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:49.850533+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |