Overview
- External ontology
- TOGO:M2746 — Eubacterium acidaminophilum medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2746 Original URL: https://www.atcc.org/~/media/56DD9004C375480F86D3CA21367B1FF5.ashx
Composition
flowchart LR
CultureMech_009297["eubacterium_acidaminophilum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009297 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009297 --> ing1_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2___2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_009297 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009297 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2___6H2O["MgCl2 . 6H2O"]
CultureMech_009297 --> ing4_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2___6H2O -.-> CHEBI_86345
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009297 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Nitrogen_gas["Nitrogen gas"]
CultureMech_009297 --> ing6_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing6_Nitrogen_gas -.-> CHEBI_17997
ing7_Biotin["Biotin"]
CultureMech_009297 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009297 --> ing8_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing8_p_Aminobenzoic_acid -.-> CHEBI_30753
ing9_Na2SeO3___5H2O["Na2SeO3 . 5H2O"]
CultureMech_009297 --> ing9_Na2SeO3___5H2O
CHEBI_48843["CHEBI:48843"]
ing9_Na2SeO3___5H2O -.-> CHEBI_48843
ing10_NaOH["NaOH"]
CultureMech_009297 --> ing10_NaOH
CHEBI_32145["CHEBI:32145"]
ing10_NaOH -.-> CHEBI_32145
ing11_N2["N2"]
CultureMech_009297 --> ing11_N2
ing11_N2 -.-> CHEBI_17997
ing12_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009297 --> ing12_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4___2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_009297 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009297 --> ing14_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2___4H2O -.-> CHEBI_86368
ing15_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009297 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009297 --> ing16_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2___6H2O -.-> CHEBI_34887
ing17_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009297 --> ing17_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2___2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_009297 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_Hydrochloric_acid__25_["Hydrochloric acid, 25%"]
CultureMech_009297 --> ing19_Hydrochloric_acid__25_
ing20_FeCl2___4H2O["FeCl2 . 4H2O"]
CultureMech_009297 --> ing20_FeCl2___4H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2___4H2O -.-> CHEBI_86249
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1068.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 966.0, 100.0, 1.0, 1.0] |
| 2 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgCl2 . 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 7 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
4 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Na2SeO3 . 5H2O
2Na.O3Se 172.937 g/mol
|
30 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 12 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | Hydrochloric acid, 25% | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Solution | |
| 21 |
FeCl2 . 4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace Element Solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
NaHCO3 solution (20% w/v)
Dose: 20 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Na2S . 9H2O solution (10% w/v)
Dose: 3 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
Vitamin Solution
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Sodium Selenite Solution
Dose: 10 G_PER_L
Na2CO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
HCl solution
Dose: variable VARIABLE
Role: Solvating media; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.222012Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2746 |
| 2026-01-28T19:48:32.111188Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Na2CO3 solution; Added default concentration for ingredient: HCl solution; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:53.311154+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:24.771764Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:05.350888Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009297 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.250833+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Eubacterium acidaminophilum medium" to "eubacterium_acidaminophilum_medium" for consistent matching |
| 2026-05-16T09:03:31.582912+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:59.719266+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.742651+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |