Overview
- External ontology
- TOGO:M2749 — Acetobacterium medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2749 Original URL: https://www.atcc.org/~/media/BFCCC10871CB40E8A84CDDC2504F7A2E.ashx
Composition
flowchart LR
CultureMech_009299["acetobacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009299 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009299 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009299 --> ing2_Yeast_extract
ing3_KH2PO4["KH2PO4"]
CultureMech_009299 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009299 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_009299 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009299 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na2S___9H2O["Na2S . 9H2O"]
CultureMech_009299 --> ing7_Na2S___9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S___9H2O -.-> CHEBI_76209
ing8_NaHCO3["NaHCO3"]
CultureMech_009299 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Fructose["Fructose"]
CultureMech_009299 --> ing9_Fructose
CHEBI_28645["CHEBI:28645"]
ing9_Fructose -.-> CHEBI_28645
ing10_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009299 --> ing10_L_Cysteine___HCl
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009299 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_009299 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_5__Na2CO3["5% Na2CO3"]
CultureMech_009299 --> ing13_5__Na2CO3
ing14_Biotin["Biotin"]
CultureMech_009299 --> ing14_Biotin
CHEBI_15956["CHEBI:15956"]
ing14_Biotin -.-> CHEBI_15956
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009299 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Thiamine___HCl["Thiamine . HCl"]
CultureMech_009299 --> ing16_Thiamine___HCl
ing17_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_009299 --> ing17_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_D_____pantothenate -.-> CHEBI_31345
ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009299 --> ing18_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing19_Folic_acid["Folic acid"]
CultureMech_009299 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_009299 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_Riboflavin["Riboflavin"]
CultureMech_009299 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_009299 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Thioctic_acid["Thioctic acid"]
CultureMech_009299 --> ing23_Thioctic_acid
CHEBI_30314["CHEBI:30314"]
ing23_Thioctic_acid -.-> CHEBI_30314
ing24_NaCl["NaCl"]
CultureMech_009299 --> ing24_NaCl
CHEBI_26710["CHEBI:26710"]
ing24_NaCl -.-> CHEBI_26710
ing25_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009299 --> ing25_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing25_Na2MoO4___2H2O -.-> CHEBI_75213
ing26_H3BO3["H3BO3"]
CultureMech_009299 --> ing26_H3BO3
CHEBI_33118["CHEBI:33118"]
ing26_H3BO3 -.-> CHEBI_33118
ing27_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009299 --> ing27_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing27_FeSO4___7H2O -.-> CHEBI_75836
ing28_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009299 --> ing28_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing28_CoCl2___6H2O -.-> CHEBI_35696
ing29_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009299 --> ing29_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing29_ZnSO4___7H2O -.-> CHEBI_32312
CultureMech_009299 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.1, 3.0] |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Fructose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 11 | L-Cysteine . HCl | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 | 5% Na2CO3 | variable VARIABLE | Role: Buffer; Properties: Defined component, Inorganic compound, Solution | |
| 15 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 | Thiamine . HCl | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 18 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 25 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
AlK(SO4)2 . 12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 34 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Wolfe's Mineral Solution
Dose: 20 G_PER_L
See mediadive_3952_Wolfe_s_mineral_solution.yaml for composition
Wolfe's Vitamin Solution
Dose: 20 G_PER_L
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.229214Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2749 |
| 2026-01-28T19:48:42.458970Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: 5% Na2CO3; Added default concentration for ingredient: KOH solution |
| 2026-02-06T05:10:54.770217Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:05:11.553879+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:43.386410Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:05.390476Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009299 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.287547+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acetobacterium medium" to "acetobacterium_medium" for consistent matching |
| 2026-05-16T09:03:15.816694+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.093983+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:28.580891+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.869404+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:47.940740+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |