Overview
- External ontology
- TOGO:M2750 — PY + X Medium (N2/CO2)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2750 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104c.pdf
Composition
flowchart LR
CultureMech_009301["py_x_medium_n2_co2"]
ing0_Distilled_water["Distilled water"]
CultureMech_009301 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009301 --> ing1_Yeast_extract
ing2_D_Glucose["D-Glucose"]
CultureMech_009301 --> ing2_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_Glucose -.-> CHEBI_17634
ing3_Na2CO3["Na2CO3"]
CultureMech_009301 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Peptone_from_meat__pepsin_digested_["Peptone from meat (pepsin-digested)"]
CultureMech_009301 --> ing4_Peptone_from_meat__pepsin_digested_
ing5_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD BBL)"]
CultureMech_009301 --> ing5_Trypticase_peptone__BD_BBL_
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
CultureMech_009301 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009301 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_009301 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009301 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_NaCl["NaCl"]
CultureMech_009301 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009301 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_KH2PO4["KH2PO4"]
CultureMech_009301 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_K2HPO4["K2HPO4"]
CultureMech_009301 --> ing13_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing13_K2HPO4 -.-> CHEBI_131527
ing14_NaHCO3["NaHCO3"]
CultureMech_009301 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Collapsed 2 identical duplicates: 1000.0, 1000.0] |
| 2 | Yeast extract | 10 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
2.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 | Peptone from meat (pepsin-digested) | 5 G_PER_L | Role: Nutrient source; Properties: Defined component | |
| 6 | Trypticase peptone (BD BBL) | 5 G_PER_L | Properties: Undefined component | |
| 7 |
L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 14 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Salt solution
Dose: 40 G_PER_L
See mediadive_6218_Salt_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.233578Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2750 |
| 2026-01-28T19:48:19.906354Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:33.006479+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:02.357288Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:05.413001Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009301 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.311326+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY + X Medium (N2/CO2)" to "py_x_medium_n2_co2" for consistent matching |
| 2026-05-16T09:03:15.843348+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.119709+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:28.608440+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.882102+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:47.957604+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |