Overview
- External ontology
- TOGO:M2752 — Anaerotignum Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2752 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium156.pdf
Composition
flowchart LR
CultureMech_009303["anaerotignum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009303 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009303 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009303 --> ing2_Yeast_extract
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009303 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_NaHCO3["NaHCO3"]
CultureMech_009303 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Potassium_phosphate_buffer__1_M__pH_7_1_["Potassium phosphate buffer (1 M, pH 7.1)"]
CultureMech_009303 --> ing5_Potassium_phosphate_buffer__1_M__pH_7_1_
ing6_L_Alanine["L-Alanine"]
CultureMech_009303 --> ing6_L_Alanine
CHEBI_16977["CHEBI:16977"]
ing6_L_Alanine -.-> CHEBI_16977
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine-HCl x H2O"]
CultureMech_009303 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_Peptone["Peptone"]
CultureMech_009303 --> ing8_Peptone
ing9_N2_gas["N2 gas"]
CultureMech_009303 --> ing9_N2_gas
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
18 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 6 | Potassium phosphate buffer (1 M, pH 7.1) | 5 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound, Complex component | |
| 7 |
L-Alanine
C3H7NO2 89.094 g/mol
|
3 G_PER_L |
CHEBI:16977
L-alanine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 8 |
L-Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 9 | Peptone | 3 G_PER_L | ||
| 10 | N2 gas | variable VARIABLE | Properties: Gas |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
CaSO4 (saturated aq. solution)
Dose: 2.5 G_PER_L
Role: Mineral source; Properties: Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.237352Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2752 |
| 2026-01-28T19:48:41.562297Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:05:10.120903+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:05.428068Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009303 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.327493+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Anaerotignum Medium" to "anaerotignum_medium" for consistent matching |
| 2026-05-16T09:03:15.852662+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.129202+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:28.618800+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |