Overview
- External ontology
- TOGO:M2758 — Reinforced Clostridial Medium (RCM) (Difco)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2758
Composition
flowchart LR
CultureMech_009308["reinforced_clostridial_medium_rcm_difco"]
ing0_Distilled_water["Distilled water"]
CultureMech_009308 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009308 --> ing1_Yeast_extract
ing2_Sodium_chloride["Sodium chloride"]
CultureMech_009308 --> ing2_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing2_Sodium_chloride -.-> CHEBI_26710
ing3_Dextrose["Dextrose"]
CultureMech_009308 --> ing3_Dextrose
CHEBI_17634["CHEBI:17634"]
ing3_Dextrose -.-> CHEBI_17634
ing4_Sodium_acetate["Sodium acetate"]
CultureMech_009308 --> ing4_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Sodium_acetate -.-> CHEBI_32954
ing5_Soluble_starch["Soluble starch"]
CultureMech_009308 --> ing5_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing5_Soluble_starch -.-> CHEBI_28017
ing6_Agar["Agar"]
CultureMech_009308 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Cysteine_HCl["Cysteine HCl"]
CultureMech_009308 --> ing7_Cysteine_HCl
ing8_Beef_extract["Beef extract"]
CultureMech_009308 --> ing8_Beef_extract
ing9_Peptone["Peptone"]
CultureMech_009308 --> ing9_Peptone
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 3 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Sodium chloride
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
Dextrose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 5 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 6 | Soluble starch | 1 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 7 | Agar | 0.5 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Cysteine HCl | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 9 | Beef extract | 10 G_PER_L | Properties: Undefined component | |
| 10 | Peptone | 10 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.250274Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2758 |
| 2026-01-28T19:48:09.379643Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:05.487246Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009308 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.393070+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Reinforced Clostridial Medium (RCM) (Difco)" to "reinforced_clostridial_medium_rcm_difco" for consistent matching |
| 2026-06-05T07:41:44.153166+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |