de_man_rogosa_sharpe_mrs_medium_scharlau_chemie_supplemented_with_0_05_wt_vol_l_cysteine_hydrochloride

CultureMech:009325 bacterial COMPLEX LIQUID
Original name: de Man-Rogosa-Sharpe (MRS) medium (Scharlau Chemie) supplemented with 0.05% (wt/vol) l-cysteine hydrochloride

Overview

External ontology
TOGO:M2777 — de Man-Rogosa-Sharpe (MRS) medium (Scharlau Chemie) supplemented with 0.05% (wt/vol) l-cysteine hydrochloride
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2777

Composition

flowchart LR
    CultureMech_009325["de_man_rogosa_sharpe_mrs_medium_scharlau_chemie_supplemented_with_0..."]
    ing0_L_cysteine_hydrochloride["L-cysteine hydrochloride"]
    CultureMech_009325 --> ing0_L_cysteine_hydrochloride
    ing1_MRS_medium__Scharlau_Chemie_["MRS medium (Scharlau Chemie)"]
    CultureMech_009325 --> ing1_MRS_medium__Scharlau_Chemie_
    ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009325 --> ing2_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing2_Carbon_dioxide_gas -.-> CHEBI_16526
    ing3_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009325 --> ing3_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing3_Nitrogen_gas -.-> CHEBI_17997
    ing4_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009325 --> ing4_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing4_Hydrogen_gas -.-> CHEBI_18276
    ing5_Distilled_water["Distilled water"]
    CultureMech_009325 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_009325 --> ing6_Yeast_extract
    ing7_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_009325 --> ing7_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing7_Dipotassium_phosphate -.-> CHEBI_131527
    ing8_D____Glucose["D(+)-Glucose"]
    CultureMech_009325 --> ing8_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing8_D____Glucose -.-> CHEBI_17634
    ing9_Sodium_acetate["Sodium acetate"]
    CultureMech_009325 --> ing9_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing9_Sodium_acetate -.-> CHEBI_32954
    ing10_Polysorbate_80["Polysorbate 80"]
    CultureMech_009325 --> ing10_Polysorbate_80
    CHEBI_53426["CHEBI:53426"]
    ing10_Polysorbate_80 -.-> CHEBI_53426
    ing11_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_009325 --> ing11_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing11_Magnesium_sulfate -.-> CHEBI_32599
    ing12_Triammonium_citrate["Triammonium citrate"]
    CultureMech_009325 --> ing12_Triammonium_citrate
    CHEBI_63037["CHEBI:63037"]
    ing12_Triammonium_citrate -.-> CHEBI_63037
    ing13_Manganese_sulfate["Manganese sulfate"]
    CultureMech_009325 --> ing13_Manganese_sulfate
    CHEBI_86364["CHEBI:86364"]
    ing13_Manganese_sulfate -.-> CHEBI_86364
    ing14_Meat_extract["Meat extract"]
    CultureMech_009325 --> ing14_Meat_extract
    ing15_Peptone_proteose["Peptone proteose"]
    CultureMech_009325 --> ing15_Peptone_proteose

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 L-cysteine hydrochloride 0.05 G_PER_L Properties: Defined component, Simple component, Organic compound
2 MRS medium (Scharlau Chemie) 1 G_PER_L
3 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
4 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
5 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
6 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
7 Yeast extract 4 G_PER_L Role: Nutrient source; Properties: Undefined component
8 Dipotassium phosphate
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
9 D(+)-Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17634
D-glucose
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
10 Sodium acetate
C2H3O2.Na 82.034 g/mol
5 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Simple component, Defined component, Organic compound
11 Polysorbate 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Simple component, Organic compound, Defined component
12 Magnesium sulfate
Mg.O4S 120.368 g/mol
0.2 G_PER_L CHEBI:32599
magnesium sulfate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
13 Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
2 G_PER_L CHEBI:63037
triammonium citrate
 Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
14 Manganese sulfate
H2O.Mn.O4S 169.016 g/mol
0.05 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
15 Meat extract 8 G_PER_L Properties: Undefined component
16 Peptone proteose 10 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.284927Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2777
2026-01-28T19:48:13.868382Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-13T06:20:05.648542Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009325
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.558168+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "de Man-Rogosa-Sharpe (MRS) medium (Scharlau Chemie) supplemented with 0.05% (wt/vol) l-cysteine hydrochloride" to "de_man_rogosa_sharpe_mrs_medium_scharlau_chemie_supplemented_with_0_05_wt_vol_l_cysteine_hydrochloride" for consistent matching
2026-05-16T09:03:28.605811+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:55.359042+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.