hayflick_medium

CultureMech:009333 bacterial COMPLEX LIQUID
Original name: Hayflick medium

Overview

External ontology
TOGO:M2785 — Hayflick medium
Applications
  • Microbial cultivation
pH range
{'min': 7.6, 'max': 8.0}
Notes
TOGO M2785 lists Hayflick medium as, per liter, 19.6 g Yeast Extract, 17.7 g PPLO annotated as Difco PPLO Broth, 0.24 g DNA sodium salt from Sigma D1501, 24 mg Phenol Red, 843 ml DI Water, and 157 ml Horse serum. The source adjusts the base to pH 7.8 +/- 0.2, autoclaves it at 121 C, cools it to 55 C, and then adds Horse serum aseptically.

Composition

flowchart LR
    CultureMech_009333["hayflick_medium"]
    ing0_Yeast_Extract["Yeast Extract"]
    CultureMech_009333 --> ing0_Yeast_Extract
    ing1_Horse_serum["Horse serum"]
    CultureMech_009333 --> ing1_Horse_serum
    ing2_Phenol_Red["Phenol Red"]
    CultureMech_009333 --> ing2_Phenol_Red
    CHEBI_31991["CHEBI:31991"]
    ing2_Phenol_Red -.-> CHEBI_31991
    ing3_Deionized_water["Deionized water"]
    CultureMech_009333 --> ing3_Deionized_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Deionized_water -.-> CHEBI_15377
    ing4_DNA_sodium_salt__Sigma__D1501_["DNA sodium salt (Sigma, D1501)"]
    CultureMech_009333 --> ing4_DNA_sodium_salt__Sigma__D1501_
    ing5_Difco_PPLO_Broth["Difco PPLO Broth"]
    CultureMech_009333 --> ing5_Difco_PPLO_Broth

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast Extract 19.6 G_PER_L FOODON:03315426
yeast extract
 TOGO M2785 lists 19.6 g/L Yeast Extract.
2 Horse serum 157.0 ML_PER_L TOGO M2785 lists 157 ml/L Horse serum, added aseptically after the autoclaved base cools to 55 C; this complex serum is retained as an opaque component.
3 Phenol Red
C19H14O5S 354.383 g/mol
24.0 MG_PER_L CHEBI:31991
phenol red
 TOGO M2785 lists 24 mg/L Phenol Red.
4 Deionized water
H2O 18.015 g/mol
843.0 ML_PER_L CHEBI:15377
water
 TOGO M2785 lists 843 ml/L DI Water.
5 DNA sodium salt (Sigma, D1501) 0.24 G_PER_L TOGO M2785 lists 0.24 g/L DNA sodium salt from Sigma catalog D1501; this calf-thymus DNA product is retained as an opaque component.
6 Difco PPLO Broth 17.7 G_PER_L TOGO M2785 lists 17.7 g/L PPLO, annotated by TOGO as Difco PPLO Broth; this catalog broth is retained as an opaque component.

Preparation

  1. Combine all ingredients except horse serum.
  2. Adjust to pH 7.8 +/- 0.2.
  3. Autoclave the base at 121 C and let the medium cool to 55 C.
  4. Aseptically add horse serum and dispense as required.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.298279Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2785
2026-01-28T19:48:08.704043Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:05.704677Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009333
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.619623+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Hayflick medium" to "hayflick_medium" for consistent matching
2026-06-05T07:41:08.325234+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2785_hayflick_score15.py RESOLVED_TOGO_M2785_SCORE15 TOGO M2785 lists Hayflick medium as, per liter, 19.6 g Yeast Extract, 17.7 g PPLO annotated as Difco PPLO Broth, 0.24 g DNA sodium salt from Sigma D1501, 24 mg Phenol Red, 843 ml DI Water, and 157 ml Horse serum. The source adjusts the base to pH 7.8 +/- 0.2, autoclaves it at 121 C, cools it to 55 C, and then adds Horse serum aseptically. Corrected the ml/L and mg/L import artifacts, added the source pH and temperature, grounded yeast extract, phenol red, and water, and kept Horse serum, DNA sodium salt, and Difco PPLO Broth unmapped as complex inputs.