Overview
- External ontology
- TOGO:M2790 — M9 Minimal Media
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2790
Composition
flowchart LR
CultureMech_009338["m9_minimal_media"]
ing0_1M_CaCl2["1M CaCl2"]
CultureMech_009338 --> ing0_1M_CaCl2
ing1_1M_MgSO4["1M MgSO4"]
CultureMech_009338 --> ing1_1M_MgSO4
ing2_20__glucose["20% glucose"]
CultureMech_009338 --> ing2_20__glucose
ing3_2__casamino_acid["2% casamino acid"]
CultureMech_009338 --> ing3_2__casamino_acid
ing4_Distilled_water["Distilled water"]
CultureMech_009338 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_x5_M9_salt__Sigma_["x5 M9 salt (Sigma)"]
CultureMech_009338 --> ing5_x5_M9_salt__Sigma_
ing6_NaCl["NaCl"]
CultureMech_009338 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_KH2PO4["KH2PO4"]
CultureMech_009338 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_NH4Cl["NH4Cl"]
CultureMech_009338 --> ing8_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing8_NH4Cl -.-> CHEBI_31206
ing9_Na2HPO4["Na2HPO4"]
CultureMech_009338 --> ing9_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing9_Na2HPO4 -.-> CHEBI_34683
ing10_H3BO3["H3BO3"]
CultureMech_009338 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_FeSO4_7H2O["FeSO4.7H2O"]
CultureMech_009338 --> ing11_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_7H2O -.-> CHEBI_75836
ing12_CuCl2_2H2O["CuCl2.2H2O"]
CultureMech_009338 --> ing12_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_2H2O -.-> CHEBI_86318
ing13_ZnCl2["ZnCl2"]
CultureMech_009338 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_MnSO4_H2O["MnSO4.H2O"]
CultureMech_009338 --> ing14_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_H2O -.-> CHEBI_86364
ing15_Co_NO3_2_6H2O["Co(NO3)2.6H2O"]
CultureMech_009338 --> ing15_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing15_Co_NO3_2_6H2O -.-> CHEBI_86214
ing16_Ca_NO3_2["Ca(NO3)2"]
CultureMech_009338 --> ing16_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing16_Ca_NO3_2 -.-> CHEBI_64205
ing17__NH4_6Mo7O24_4H2O["(NH4)6Mo7O24.4H2O"]
CultureMech_009338 --> ing17__NH4_6Mo7O24_4H2O
CHEBI_91249["CHEBI:91249"]
ing17__NH4_6Mo7O24_4H2O -.-> CHEBI_91249
ing18_NH4VO3["NH4VO3"]
CultureMech_009338 --> ing18_NH4VO3
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 1M CaCl2 | 10 G_PER_L | Role: Mineral source; Properties: Solution, Inorganic compound, Defined component | |
| 2 | 1M MgSO4 | 200 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component | |
| 3 | 20% glucose | 2.5 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Solution | |
| 4 | 2% casamino acid | 10 G_PER_L | Properties: Undefined component, Solution | |
| 5 |
Distilled water
H2O 18.015 g/mol
|
102.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 100.0, 1.0, 1.0] |
| 6 | x5 M9 salt (Sigma) | 20 G_PER_L | ||
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
15 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NH4Cl
Cl.H4N 53.492 g/mol
|
5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
33.9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuCl2.2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.25 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.25 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnSO4.H2O
H2O.Mn.O4S 169.016 g/mol
|
1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Co(NO3)2.6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.25 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
2.5 G_PER_L |
CHEBI:64205
calcium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
(NH4)6Mo7O24.4H2O
2H4N.MoO4 196.014 g/mol
|
0.25 G_PER_L |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 | NH4VO3 | 0.1 G_PER_L | Role: Mineral source |
Solutions
Trace elements
Dose: 100 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.304287Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2790 |
| 2026-01-28T19:48:36.652691Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:01.138443+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:32.969243Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:05.752634Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009338 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.668072+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M9 Minimal Media" to "m9_minimal_media" for consistent matching |
| 2026-05-16T09:03:39.605771+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:12.005526+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.508909+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:00.718609+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |