blood_agar_tsa_with_5_sheep_blood_remel

CultureMech:009339 bacterial COMPLEX SOLID_AGAR
Original name: Blood agar (TSA with 5% sheep blood) (Remel)

Overview

External ontology
TOGO:M2791 — Blood agar (TSA with 5% sheep blood) (Remel)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2791 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_009339["blood_agar_tsa_with_5_sheep_blood_remel"]
    ing0_demineralized_water["demineralized water"]
    CultureMech_009339 --> ing0_demineralized_water
    CHEBI_15377["CHEBI:15377"]
    ing0_demineralized_water -.-> CHEBI_15377
    ing1_Sheep_blood["Sheep blood"]
    CultureMech_009339 --> ing1_Sheep_blood
    ing2_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_009339 --> ing2_Pancreatic_digest_of_casein
    ing3_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_009339 --> ing3_Peptic_digest_of_soybean_meal
    ing4_Glucose["Glucose"]
    CultureMech_009339 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Sodium_chloride["Sodium chloride"]
    CultureMech_009339 --> ing5_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_chloride -.-> CHEBI_26710
    ing6_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_009339 --> ing6_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing6_Dipotassium_phosphate -.-> CHEBI_131527
    ing7_Agar["Agar"]
    CultureMech_009339 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_CO2["CO2"]
    CultureMech_009339 --> ing8_CO2
    CHEBI_16526["CHEBI:16526"]
    ing8_CO2 -.-> CHEBI_16526

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 demineralized water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Sheep blood 5 PERCENT_W_V Role: Nutrient source; Properties: Undefined component
3 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
4 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
5 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
6 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
7 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
8 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
9 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: Gas

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.306792Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2791
2026-01-28T19:48:13.178133Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2
2026-03-13T06:20:05.758493Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009339
2026-03-17T06:29:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.674550+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Blood agar (TSA with 5% sheep blood) (Remel)" to "blood_agar_tsa_with_5_sheep_blood_remel" for consistent matching
2026-05-16T09:03:25.927472+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:51.168242+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.