mrs_broth_criterion_usa_containing_0_5_l_cysteine_sigma_usa

CultureMech:009344 bacterial COMPLEX LIQUID
Original name: MRS broth (Criterion, USA) containing 0.5%L-cysteine (Sigma, USA)

Overview

External ontology
TOGO:M2796 — MRS broth (Criterion, USA) containing 0.5%L-cysteine (Sigma, USA)
Applications
  • Microbial cultivation
pH range
{'min': 6.3, 'max': 6.7}
Notes
TOGO M2796 lists 55.0 g/L MRS broth from Criterion with 0.5% w/v L-cysteine from Sigma in 1.0 L distilled water at pH 6.5 +/- 0.2.

Composition

flowchart LR
    CultureMech_009344["mrs_broth_criterion_usa_containing_0_5_l_cysteine_sigma_usa"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009344 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_L_cysteine__Sigma_["L-cysteine (Sigma)"]
    CultureMech_009344 --> ing1_L_cysteine__Sigma_
    CHEBI_17561["CHEBI:17561"]
    ing1_L_cysteine__Sigma_ -.-> CHEBI_17561
    ing2_MRS_broth__Criterion_["MRS broth (Criterion)"]
    CultureMech_009344 --> ing2_MRS_broth__Criterion_

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M2796 lists 1.0 L Distilled water.
2 L-cysteine (Sigma)
C3H7NO2S 121.161 g/mol
0.5 PERCENT_W_V CHEBI:17561
L-cysteine
 TOGO M2796 lists 0.5 % w/v L-cysteine (Sigma).
3 MRS broth (Criterion) 55.0 G_PER_L TOGO M2796 lists 55.0 g/L MRS broth (Criterion).

Preparation

  1. Dissolve 55.0 g MRS broth (Criterion) and 0.5% w/v L-cysteine in 1.0 L distilled water, then adjust pH to 6.5 +/- 0.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.310737Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2796
2026-01-28T19:48:09.124404Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:05.773212Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009344
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.690643+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS broth (Criterion, USA) containing 0.5%L-cysteine (Sigma, USA)" to "mrs_broth_criterion_usa_containing_0_5_l_cysteine_sigma_usa" for consistent matching
2026-06-05T07:41:38.442975+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m2242_m2796_m2804_mrs_broths_score15.py RESOLVED_TOGO_M2796_MRS_CRITERION_SCORE15 TOGO M2796 lists 55.0 g/L MRS broth from Criterion with 0.5% w/v L-cysteine from Sigma in 1.0 L distilled water at pH 6.5 +/- 0.2. Corrected imported one-liter water units, normalized source gram and milliliter quantities, added the source pH range, and grounded unambiguous components.