Overview
- External ontology
- TOGO:M2803 — de Man-Rogosa-Sharpe broths supplemented with 0.05% l-cysteine · HCl
- Applications
-
- Microbial cultivation
- Notes
- TOGO M2803 records Difco MRS broth supplemented to a final 0.05% L-cysteine HCl concentration and cultivated at 37 C under anaerobic BBL Anaerobic system conditions; the source does not disclose the MRS product's internal formulation.
Composition
flowchart LR
CultureMech_009351["de_man_rogosa_sharpe_broths_supplemented_with_0_05_l_cysteine_hcl"]
ing0_MRS_broth__Difco_["MRS broth (Difco)"]
CultureMech_009351 --> ing0_MRS_broth__Difco_
ing1_L_cysteine___HCl["L-cysteine · HCl"]
CultureMech_009351 --> ing1_L_cysteine___HCl
CHEBI_91247["CHEBI:91247"]
ing1_L_cysteine___HCl -.-> CHEBI_91247
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | MRS broth (Difco) | 1000 ML_PER_L | TOGO M2803 lists one liter of MRS broth from Difco; TOGO M2489 represents the same opaque product. | |
| 2 |
L-cysteine · HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.05 PERCENT_W_V |
CHEBI:91247
L-cysteine hydrochloride
|
TOGO M2803 lists L-cysteine HCl from Sigma at a 0.05% final concentration. |
Preparation
- Supplement one liter of MRS broth from Difco with L-cysteine HCl to a 0.05% final concentration.
- Cultivate under anaerobic BBL Anaerobic system conditions at 37 C.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.315404Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2803 |
| 2026-03-13T06:20:05.808577Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009351 |
| 2026-03-17T19:46:24.731537+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "de Man-Rogosa-Sharpe broths supplemented with 0.05% l-cysteine · HCl" to "de_man_rogosa_sharpe_broths_supplemented_with_0_05_l_cysteine_hcl" for consistent matching |
| 2026-09-08T00:00:00-07:00 | repair_togo_mrs_marine_urine_score40.py | RESOLVED_TOGO_MRS_MARINE_URINE_SCORE40 | TOGO M2803 records Difco MRS broth supplemented to a final 0.05% L-cysteine HCl concentration and cultivated at 37 C under anaerobic BBL Anaerobic system conditions; the source does not disclose the MRS product's internal formulation. |