Overview
- External ontology
- TOGO:M2812 — Brain heart infusion broth supplemented with 5 μg/ml hemin, 1 g/L‐cysteine, and NaHCO3 (BHIS)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2812 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009360["brain_heart_infusion_broth_supplemented_with_5_g_ml_hemin_1_g_l_cys..."]
ing0_NaHCO3["NaHCO3"]
CultureMech_009360 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Hemin["Hemin"]
CultureMech_009360 --> ing1_Hemin
CHEBI_50385["CHEBI:50385"]
ing1_Hemin -.-> CHEBI_50385
ing2_Cysteine["Cysteine"]
CultureMech_009360 --> ing2_Cysteine
CHEBI_15356["CHEBI:15356"]
ing2_Cysteine -.-> CHEBI_15356
ing3_Calf_brains["Calf brains"]
CultureMech_009360 --> ing3_Calf_brains
ing4_Beef_heart["Beef heart"]
CultureMech_009360 --> ing4_Beef_heart
ing5_Proteose_peptone["Proteose peptone"]
CultureMech_009360 --> ing5_Proteose_peptone
ing6_Dextrose["Dextrose"]
CultureMech_009360 --> ing6_Dextrose
CHEBI_17634["CHEBI:17634"]
ing6_Dextrose -.-> CHEBI_17634
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_009360 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Disodium_phosphate["Disodium phosphate"]
CultureMech_009360 --> ing8_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing8_Disodium_phosphate -.-> CHEBI_34683
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
5 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Defined component, Organic compound, Simple component |
| 3 |
Cysteine
C3H7NO2S 121.161 g/mol
|
1 G_PER_L |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component |
| 4 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 5 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 6 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 7 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 9 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.326679Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2812 |
| 2026-01-28T19:48:07.150940Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:05.868099Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009360 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.794123+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Brain heart infusion broth supplemented with 5 μg/ml hemin, 1 g/L‐cysteine, and NaHCO3 (BHIS)" to "brain_heart_infusion_broth_supplemented_with_5_g_ml_hemin_1_g_l_cysteine_and_nahco3_bhis" for consistent matching |
| 2026-06-05T07:40:51.500906+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |