dt_medium_ph_3_5

CultureMech:009364 bacterial COMPLEX LIQUID
Original name: DT medium (pH 3.5)

Overview

External ontology
TOGO:M2816 — DT medium (pH 3.5)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2816

Composition

flowchart LR
    CultureMech_009364["dt_medium_ph_3_5"]
    ing0_CaCl2___2H2O["CaCl2 · 2H2O"]
    CultureMech_009364 --> ing0_CaCl2___2H2O
    CHEBI_86158["CHEBI:86158"]
    ing0_CaCl2___2H2O -.-> CHEBI_86158
    ing1_MgSO4["MgSO4"]
    CultureMech_009364 --> ing1_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4 -.-> CHEBI_32599
    ing2_K2SO4["K2SO4"]
    CultureMech_009364 --> ing2_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing2_K2SO4 -.-> CHEBI_32036
    ing3_dextrin["dextrin"]
    CultureMech_009364 --> ing3_dextrin
    CHEBI_28675["CHEBI:28675"]
    ing3_dextrin -.-> CHEBI_28675
    ing4_NaH2PO4["NaH2PO4"]
    CultureMech_009364 --> ing4_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing4_NaH2PO4 -.-> CHEBI_37585
    ing5_EZMix_N_Z_amine_A["EZMix-N-Z-amine A"]
    CultureMech_009364 --> ing5_EZMix_N_Z_amine_A
    ing6_MnCl2___4H2O["MnCl2 · 4H2O"]
    CultureMech_009364 --> ing6_MnCl2___4H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2___4H2O -.-> CHEBI_86368
    ing7_CoCl2___6H2O["CoCl2 · 6H2O"]
    CultureMech_009364 --> ing7_CoCl2___6H2O
    CHEBI_35696["CHEBI:35696"]
    ing7_CoCl2___6H2O -.-> CHEBI_35696
    ing8_CuCl2___2H2O["CuCl2 · 2H2O"]
    CultureMech_009364 --> ing8_CuCl2___2H2O
    CHEBI_86318["CHEBI:86318"]
    ing8_CuCl2___2H2O -.-> CHEBI_86318
    ing9_ZnCl2["ZnCl2"]
    CultureMech_009364 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_FeCl3["FeCl3"]
    CultureMech_009364 --> ing10_FeCl3
    CHEBI_30808["CHEBI:30808"]
    ing10_FeCl3 -.-> CHEBI_30808

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4
Mg.O4S 120.368 g/mol
0.145 G_PER_L CHEBI:32599
magnesium sulfate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 K2SO4
2K.O4S 174.259 g/mol
3 G_PER_L CHEBI:32036
potassium sulfate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 dextrin 0.1 G_PER_L CHEBI:28675
dextrin
Role: Carbon source; Properties: Simple component, Organic compound, Defined component
5 NaH2PO4
H2O4P.Na 119.976 g/mol
0.5 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
6 EZMix-N-Z-amine A 0.1 G_PER_L
7 MnCl2 · 4H2O
Cl2Mn.4H2O 197.904 g/mol
0.5 PERCENT_W_V CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8 CoCl2 · 6H2O
2Cl.Co 129.839 g/mol
0.5 PERCENT_W_V CHEBI:35696
cobalt dichloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 CuCl2 · 2H2O
Cl2Cu.2H2O 170.482 g/mol
0.5 PERCENT_W_V CHEBI:86318
copper(II) chloride dihydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 ZnCl2
2Cl.Zn 136.296 g/mol
0.5 PERCENT_W_V CHEBI:49976
zinc dichloride
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
11 FeCl3
Cl3Fe 162.204 g/mol
3 PERCENT_W_V CHEBI:30808
iron trichloride
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component

Solutions

trace mineral stock solution

Dose: 20 G_PER_L

See mediadive_5478_Trace_mineral_stock_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.330523Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2816
2026-01-28T19:48:28.908474Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:47.936288+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:05.886744Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009364
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:26.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.813974+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DT medium (pH 3.5)" to "dt_medium_ph_3_5" for consistent matching
2026-05-16T09:03:31.175601+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:59.148136+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.569308+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.