Overview
- External ontology
- TOGO:M2825 — basal medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2825
Composition
flowchart LR
CultureMech_009371["basal_medium"]
ing0_thiamine["thiamine"]
CultureMech_009371 --> ing0_thiamine
CHEBI_18385["CHEBI:18385"]
ing0_thiamine -.-> CHEBI_18385
ing1_rabbit_serum["rabbit serum"]
CultureMech_009371 --> ing1_rabbit_serum
ing2_5_fluorouracil["5-fluorouracil"]
CultureMech_009371 --> ing2_5_fluorouracil
CHEBI_46345["CHEBI:46345"]
ing2_5_fluorouracil -.-> CHEBI_46345
ing3_Na2HPO4_KH2PO4_buffer["Na2HPO4-KH2PO4 buffer"]
CultureMech_009371 --> ing3_Na2HPO4_KH2PO4_buffer
ing4_NH4CI["NH4CI"]
CultureMech_009371 --> ing4_NH4CI
ing5_distilled_water["distilled water"]
CultureMech_009371 --> ing5_distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_distilled_water -.-> CHEBI_15377
ing6_KH2PO4["KH2PO4"]
CultureMech_009371 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_Na2HPO4["Na2HPO4"]
CultureMech_009371 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
thiamine
C12H17N4OS 265.362 g/mol
|
5 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 2 | rabbit serum | 10 PERCENT_W_V | Role: Nutrient source; Properties: Undefined component | |
| 3 |
5-fluorouracil
C4H3FN2O2 130.078 g/mol
|
100 G_PER_L |
CHEBI:46345
5-fluorouracil
|
|
| 4 | Na2HPO4-KH2PO4 buffer | 0.02 MOLAR | ||
| 5 | NH4CI | variable VARIABLE | Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component | |
| 6 |
distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.337954Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2825 |
| 2026-01-28T19:48:22.742216Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NH4CI |
| 2026-03-13T06:20:05.923871Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009371 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.852617+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "basal medium" to "basal_medium" for consistent matching |
| 2026-05-16T09:03:16.125744+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:39.393381+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.968352+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:23.829482+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |