basal_medium

CultureMech:009372 bacterial COMPLEX SOLID_AGAR
Original name: basal medium

Overview

External ontology
TOGO:M2826 — basal medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2826

Composition

flowchart LR
    CultureMech_009372["basal_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009372 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_KH2PO4["KH2PO4"]
    CultureMech_009372 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_009372 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009372 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_MgSO4["MgSO4"]
    CultureMech_009372 --> ing4_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing4_MgSO4 -.-> CHEBI_32599
    ing5_glucose["glucose"]
    CultureMech_009372 --> ing5_glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_glucose -.-> CHEBI_17234
    ing6_agar["agar"]
    CultureMech_009372 --> ing6_agar
    CHEBI_2509["CHEBI:2509"]
    ing6_agar -.-> CHEBI_2509
    ing7_CaCl2___2H2O["CaCl2 · 2H2O"]
    CultureMech_009372 --> ing7_CaCl2___2H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2___2H2O -.-> CHEBI_86158
    ing8_ZnCl2["ZnCl2"]
    CultureMech_009372 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_MnSO4___4_H2O["MnSO4 · 4 H2O"]
    CultureMech_009372 --> ing9_MnSO4___4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing9_MnSO4___4_H2O -.-> CHEBI_86358
    ing10_FeSO4_4H2O["FeSO4·4H2O"]
    CultureMech_009372 --> ing10_FeSO4_4H2O

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 KH2PO4
H2O4P.K 136.084 g/mol
6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
3 K2HPO4
HO4P.2K 174.174 g/mol
14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
4 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound
5 MgSO4
Mg.O4S 120.368 g/mol
0.2 G_PER_L CHEBI:32599
magnesium sulfate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Defined component, Simple component, Organic compound
7 agar 20 G_PER_L CHEBI:2509
agar
Role: Solidifying component
8 CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
1 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 ZnCl2
2Cl.Zn 136.296 g/mol
1 MILLIMOLAR CHEBI:49976
zinc dichloride
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 MnSO4 · 4 H2O
4H2O.Mn.O4S 223.061 g/mol
1 MILLIMOLAR CHEBI:86358
manganese(II) sulfate tetrahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
11 FeSO4·4H2O 1 MILLIMOLAR Role: Mineral source

Solutions

trace elements

Dose: 2 G_PER_L

See mediadive_6183_Trace_elements.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:57.339113Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2826
2026-01-28T19:48:09.027578Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:14.520948+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:05.930234Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009372
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.859233+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "basal medium" to "basal_medium" for consistent matching
2026-05-16T09:03:16.133455+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:39.401136+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.970985+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.