Overview
- External ontology
- TOGO:M2833 — MRS medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2833
Composition
flowchart LR
CultureMech_009377["mrs_medium"]
ing0_distilled_water["distilled water"]
CultureMech_009377 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009377 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_009377 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_polyoxyethylene_sorbitan_mono_oleate__Tween_80_["polyoxyethylene sorbitan mono-oleate (Tween 80)"]
CultureMech_009377 --> ing3_polyoxyethylene_sorbitan_mono_oleate__Tween_80_
ing4_MnSO4___4_H2O["MnSO4 · 4 H2O"]
CultureMech_009377 --> ing4_MnSO4___4_H2O
CHEBI_86358["CHEBI:86358"]
ing4_MnSO4___4_H2O -.-> CHEBI_86358
ing5_triammonium_citrate["triammonium citrate"]
CultureMech_009377 --> ing5_triammonium_citrate
CHEBI_63037["CHEBI:63037"]
ing5_triammonium_citrate -.-> CHEBI_63037
ing6_xylose["xylose"]
CultureMech_009377 --> ing6_xylose
CHEBI_18222["CHEBI:18222"]
ing6_xylose -.-> CHEBI_18222
ing7_arabinose["arabinose"]
CultureMech_009377 --> ing7_arabinose
CHEBI_22599["CHEBI:22599"]
ing7_arabinose -.-> CHEBI_22599
ing8_CH3COONa_3H2O["CH3COONa.3H2O"]
CultureMech_009377 --> ing8_CH3COONa_3H2O
ing9_glucose["glucose"]
CultureMech_009377 --> ing9_glucose
CHEBI_17234["CHEBI:17234"]
ing9_glucose -.-> CHEBI_17234
ing10_Oxoid_peptone["Oxoid peptone"]
CultureMech_009377 --> ing10_Oxoid_peptone
ing11_Lab_Lemco__Oxoid_["Lab-Lemco (Oxoid)"]
CultureMech_009377 --> ing11_Lab_Lemco__Oxoid_
ing12_Yeast_extract__Difco_or_Oxoid_["Yeast extract (Difco or Oxoid)"]
CultureMech_009377 --> ing12_Yeast_extract__Difco_or_Oxoid_
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
200 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 | polyoxyethylene sorbitan mono-oleate (Tween 80) | 1 G_PER_L | Role: Surfactant; Properties: Defined component, Simple component, Organic compound | |
| 5 |
MnSO4 · 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
50 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
triammonium citrate
C6H5O7.3H4N 243.216 g/mol
|
2 G_PER_L |
CHEBI:63037
triammonium citrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound |
| 7 |
xylose
C5H10O5 150.13 g/mol
|
6 G_PER_L |
CHEBI:18222
xylose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 8 |
arabinose
C5H10O5 150.13 g/mol
|
1 G_PER_L |
CHEBI:22599
arabinose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 9 | CH3COONa.3H2O | 5 G_PER_L | Role: Buffer; Properties: Defined component, Organic compound, Simple component | |
| 10 |
glucose
C6H12O6 180.156 g/mol
|
12 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 11 | Oxoid peptone | 10 G_PER_L | Properties: Undefined component | |
| 12 | Lab-Lemco (Oxoid) | 10 G_PER_L | Properties: Undefined component | |
| 13 | Yeast extract (Difco or Oxoid) | 5 G_PER_L | Properties: Complex component, Undefined component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.342413Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2833 |
| 2026-01-28T19:48:25.291673Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:05.951950Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009377 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.881970+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MRS medium" to "mrs_medium" for consistent matching |
| 2026-06-05T07:40:39.420291+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:28.844744+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.978470+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |