Overview
- External ontology
- TOGO:M283 — Modified MB
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M283 Original source: JCM - JCM_M289 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=289
Composition
flowchart LR
CultureMech_009381["modified_mb"]
ing0_Distilled_water["Distilled water"]
CultureMech_009381 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009381 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009381 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009381 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009381 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_009381 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009381 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_009381 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Soluble_starch["Soluble starch"]
CultureMech_009381 --> ing8_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing8_Soluble_starch -.-> CHEBI_28017
ing9_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009381 --> ing9_Yeast_extract__BD_Difco_
ing10_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009381 --> ing10_Tryptone__BD_Difco_
ing11_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009381 --> ing11_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing12_Na2S2O3["Na2S2O3"]
CultureMech_009381 --> ing12_Na2S2O3
CHEBI_32150["CHEBI:32150"]
ing12_Na2S2O3 -.-> CHEBI_32150
ing13_N2_gas["N2 gas"]
CultureMech_009381 --> ing13_N2_gas
ing14_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009381 --> ing14_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_7H2O -.-> CHEBI_31795
ing15_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009381 --> ing15_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_2H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_009381 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009381 --> ing17_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_7H2O -.-> CHEBI_75836
ing18_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009381 --> ing18_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing18_CoCl2_6H2O -.-> CHEBI_35696
ing19_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009381 --> ing19_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_7H2O -.-> CHEBI_32312
ing20_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_009381 --> ing20_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_5H2O -.-> CHEBI_31440
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009381 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_009381 --> ing22_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing22_MnSO4_xH2O -.-> CHEBI_86360
ing23_AKI_SO4_2["AKI(SO4)2"]
CultureMech_009381 --> ing23_AKI_SO4_2
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
3.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 1.0] |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15000000000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.05, 0.1] |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | Soluble starch | 10 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 10 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 11 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 12 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 13 |
Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
|
variable VARIABLE |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Solution |
| 14 | N2 gas | variable VARIABLE | Properties: Gas | |
| 15 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 23 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 | AKI(SO4)2 | 0.01 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution (see below)
Dose: 9 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.761699Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M283 |
| 2026-01-28T19:48:39.337582Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2S2O3; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:05:06.116191+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:37.913694Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:05.968299Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009381 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.897727+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified MB" to "modified_mb" for consistent matching |
| 2026-05-16T09:03:16.168022+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.432321+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:28.861590+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.981836+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:48.220144+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |