Overview
- External ontology
- TOGO:M2847 — Eagle's minimum essential medium (MEM) (containing antibiotics and fetal bovine serum)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2847
Composition
flowchart LR
CultureMech_009389["eagles_minimum_essential_medium_mem_containing_antibiotics_and_feta..."]
ing0_fetal__bovine__serum["fetal bovine serum"]
CultureMech_009389 --> ing0_fetal__bovine__serum
ing1_streptomycin["streptomycin"]
CultureMech_009389 --> ing1_streptomycin
CHEBI_17076["CHEBI:17076"]
ing1_streptomycin -.-> CHEBI_17076
ing2_amphotericin__B["amphotericin B"]
CultureMech_009389 --> ing2_amphotericin__B
CHEBI_2682["CHEBI:2682"]
ing2_amphotericin__B -.-> CHEBI_2682
ing3_Eagle_s__minimum__essential__medium___MEM___Nissui__Co____Tokyo_["Eagle's minimum essential medium (MEM) (Nissui Co., Tokyo)"]
CultureMech_009389 --> ing3_Eagle_s__minimum__essential__medium___MEM___Nissui__Co____Tokyo_
ing4_penicillin["penicillin"]
CultureMech_009389 --> ing4_penicillin
CHEBI_17334["CHEBI:17334"]
ing4_penicillin -.-> CHEBI_17334
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | fetal bovine serum | 2 PERCENT_W_V | Role: Nutrient source; Properties: Undefined component | |
| 2 |
streptomycin
C21H39N7O12 581.58 g/mol
|
150 G_PER_L |
CHEBI:17076
streptomycin
|
Role: Antimicrobial agent; Properties: Solution |
| 3 |
amphotericin B
C47H73NO17 924.091 g/mol
|
20 G_PER_L |
CHEBI:2682
amphotericin B
|
Role: Antimicrobial agent |
| 4 | Eagle's minimum essential medium (MEM) (Nissui Co., Tokyo) | 1 G_PER_L | ||
| 5 |
penicillin
C9H11N2O4SR 243.26 g/mol
|
variable VARIABLE |
CHEBI:17334
penicillin
|
Role: Antimicrobial agent; Properties: Defined component, Organic compound, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.354070Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2847 |
| 2026-01-28T19:48:37.714255Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: penicillin |
| 2026-03-13T06:20:06.007726Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009389 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.940679+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Eagle's minimum essential medium (MEM) (containing antibiotics and fetal bovine serum)" to "eagles_minimum_essential_medium_mem_containing_antibiotics_and_fetal_bovine_serum" for consistent matching |
| 2026-05-16T09:03:31.265341+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:59.273228+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.608131+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:01:43.498431+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |